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Purpose : role the sea cucumber mucopolysaccharide (SJAMP) with different concentrations of human cervical cancer cell line Hela cells to observe the impact of the SJAMP Hela cell proliferation and differentiation and to explore its possible mechanism of action , provide for research SJAMP anticancer effects theory basis. METHODS: Cultured Hela cells the different concentrations SJAMP role in Hela cells , respectively, the following studies : (1) SJAMP Hela cell proliferation inhibition observed by MTT test (MTT) ; ( 2) observed by light microscopy the Hela cells SJAMP role in the form and amount of change ; ultrastructural changes in HeLa cells under electron microscopy SJAMP role ; ( 3 ) by RT-PCR assay of cyclinD1 , CDK4 , C - myc mRNA expression . Results : (1) by MTT method showed that: the the different concentration group SJAMP comparison with the control group , OD values ??were significantly lower (except 0.5mg/ml group and the control group , P <0.05 outside other groups P <0.01 ) with the concentration increased , OD values ??reduced more significantly (P <0.01). SJAMP can effectively inhibit the proliferation of Hela cells , and this inhibition is dose-dependent ; ( 2) light microscopy showed SJAMP effect , the cell number decreased , sparsely arranged , thin volume , fusiform or round , indicating SJAMP able to significantly inhibit the proliferation of Hela cells , with the increase of the concentration of the drug , the extension of the duration of action , SJAMP proliferation of Hela cells stronger . Transmission electron microscopy revealed nucleus cytoplasm ratio decreases SJAMP role nucleosomes tend rules to shape and the nucleolus reduce or disappear , increased heterochromatin , well-differentiated organelles in the cytoplasm , The description SJAMP can induce cell differentiation to mature cells ; (3 ) RT-PCR experiments results show that , compared with control group SJAMP can significantly reduce cyclin D1, CDK4, C-myc mRNA expression , and prolonged duration of action , and the increase of the concentration of the drug , to reduce the magnitude of the more obvious . Conclusion , : SJAMP Hela cells growth inhibition and induction of differentiation , the mechanism may be to inhibit cell proliferation by inhibiting cell cycle factors cyclin D1 and CDK4 expression ; Hela cells induced to differentiate by inhibiting the expression of C-myc oncogene .
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