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The Study of Interaction Mechanism between Hepatitis A Antigen (HAAg) and Hepatitis a Antigen (HBsAg) in Combined Hepatitis A and Hepatitis B Vaccine

Author: XiongQiuXia
Tutor: ZuoJiaDai；XieZhongPing；ShiLi
School: Peking Union Medical College , China
Course: Genetics
Keywords: And B combined vaccine Cellular immune response Humoral immune responses Interaction
CLC: R392
Type: Master's thesis
Year: 2008
Downloads: 61
Quote: 0
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Abstract


Hepatitis A (HAV) and hepatitis B (HB) is the most common viral hepatitis, infectious disease is a serious danger to public health. Hepatitis B virus (hepatitis B viral) infection can develop into a chronic liver disease, cirrhosis or hepatocellular carcinoma. The hepatitis A virus (hepatitis A viral) infection usually does not cause chronic liver disease, a small number of adult patients with persistent disease or relapse, individual course of up to six months in patients with poor prognosis. Although the main route of transmission of hepatitis A and hepatitis B, but in recent years a large number of reports, a considerable number of people infected with both hepatitis A and hepatitis B hepatitis patients. Therefore, prevention of hepatitis A and hepatitis B control of viral hepatitis infection and prevalence is particularly important. Because of the hepatitis A and B is harmful to human health and no effective drug treatment for hepatitis A, hepatitis B vaccine widely used may play a very important role in containing the prevalence of the of HAV hepatitis B virus. In recent years, the development and use of containing two or more antigens, two or more joint disease vaccine preventable by the attention of the world, has become the hotspot of vaccine development. The combination vaccine for the continuous immunization programs for children, to simplify and expand the necessary. A hepatitis B combination vaccine the (Combined Hepatities A and Bvaccine) is A, hepatitis B double-specific protective properties of a combination vaccine for the prevention of these two diseases at the same time. A hepatitis B combination vaccine research A hepatitis B combination vaccine induces the body to produce higher antibody levels than the monovalent vaccines. Researchers have generally agreed that the joint vaccination induce better reaction of hepatitis B, suggesting that the two antigens may interact with each other adjuvant than Price seedlings inoculated, but no system immunology research reported. The experiment by the detection of different proportions of the experimental A hepatitis B combination vaccine and the monovalent vaccine in mice after four indicators: flow cytometry cytokine levels and T cell subsets typing, MTT assay measured spleen cell proliferation the level of the ELISA assay antibody levels to assess the cellular and humoral immune situation of the combined vaccine, as a measure of the nature of the immune response, assessing the effect of immune memory, and ultimately get the best ratio of the two vaccines, and further analysis and B jointly The two antigen interaction mechanism vaccine, but also look forward to a more comprehensive understanding of a hepatitis B combination vaccine immunogenicity. Inactivated hepatitis A vaccine produced by the Institute of Medical Biology and Shenzhen Kangtai Biological Products Company production of genetically engineered recombinant hepatitis B the yeast vaccine semi 640Eu/ml hepatitis A antigen and 10ug and 20ug hepatitis B surface antigen mixing them. Different proportions of the combined vaccine monovalent vaccine inoculation in BALB / C mice gastrocnemius and 200μl / only. Each group of five mice, four weeks after inoculation, and then the gastrocnemius injected with the same vaccine were given a booster, 200μl /. 1, 2, 3, and 4 weeks after the initial immunization and two weeks after the boost immunization, were used to detect the humoral and cellular immunity. The results showed that: a hepatitis B combination vaccine have shown that mice can stimulate produce a monovalent seedlings stronger immune response. Added hepatitis A antigen in the initial immunization, the early to a certain extent enhance the immunogenicity of the hepatitis B vaccine, and played a role of adjuvant. Comprehensive analysis of the test results, we have come to the conclusion that I independently developed a hepatitis B combination vaccine 640Eu/ml: 10μg/ml ratio on immune effect is suited to your needs. The combination vaccine can stimulate strong Th1 response in mice, Th1 and Th2 cytokines were significantly increased after booster immunization.

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CLC: > Medicine, health > Basic Medical > Medical Immunology
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