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Roles of the MAPK and MPF Activities in Fusion of Nucleolar Precursor Bodies Following Activation of Mouse Oocytes

Author: LiJingJing
Tutor: TanJingHe
School: Shandong Agricultural University
Course: Basic Veterinary
Keywords: The nucleolus precursor ( nPBS ) Merge MPF MAPK Mice Oocytes
CLC: S814
Type: Master's thesis
Year: 2009
Downloads: 34
Quote: 0
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Abstract


Nucleolus is the cells for the synthesis of ribosomal RNAs (rRNAs) , processing, and spaces of the ribosome with the protein assembly is formed . After fertilization of the oocyte , formed in prokaryotic the nucleolus precursor ( nPBS ) . Although research shows assembly of NPBs , growth and merging cytoplasmic factor depends on the the prokaryotic transcription activity and oocyte maturation process , but the M phase to G1 phase transition the nucleolar precursor fusion and motion mechanism is unclear when . Signaling pathway inhibitors / activator processing , microscopy , and protein kinase activity analysis method to study the mouse oocyte activation of MAPK and of MPF in NPBsi fusion . The main results are as follows : 1 the mouse oocytes original nuclear first formed more dispersed NPBs these NPBs then gradual integration , and eventually form a large of NPB . Alcohol activated 3.9 hours prokaryotic multiple small NPBs , fused into a big of NPB After 5.5 hours to 15.1 hours disappeared . SrCl2 for 6h , 3.8 hours after the start activating multiple small NPBs 4.5 hours fused into a big of NPB disappeared , to 18.7 hours . 3 after activation of the alcohol , 1 hour MPF activity is decreased to a minimum , and MAPK: active until after activation of 3 hours began to decline ; SrCl2 handle 6 hours after activation MPF and MAPK activity similar change occurs just the MAPK high activity is maintained for 3 hours or more . The 4 .. alcohol activated MAPK inhibitor U0126 or MPF activator MG132 treatment stop NPBs fusion , but not using the the MPF inhibitor ROS processing affect NPBs fusion . SrCl2 for 5min or six hours using the U0126 and MG132 treatment also got the same results . 5 . The alcohol and SrCl2 processing is activated at different times with U0126 and MG132 handling found , to activate during the first 2 hours MAPK high activity is maintained NPBs fusion necessary , but MPF high activity to maintain a longer fusion the worse nPBS . 6 fresh eggs SrCl2 activation process (6h) signaling pathway inhibitor / activator treatment the fusion of NPBs with hCG 18 hours after the eggs are similar . Oocytes MPF and MAPK activity in vivo in the aging process declining fusion of NPBs also declined . In short, the high activity of mouse oocytes activated MAPK at least for 2 hours , and MPF activity must immediately drop to fusion NPBs .

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > General Animal Science > Livestock and poultry breeding
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