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Environment of biological genetic material with damage induced by chemical, physical and biological factors known as genotoxicity genotoxin, including mutagens (mutagen), the chromosome clastogeny agent (clastogen) and aneuploidy inducers (aneugen) and so on. Aneuploidy-inducing agents can cause human somatic cells to become cancerous, one of the important factors of spontaneous abortion and genetic diseases. This type of genetic environment of toxic substances will undoubtedly pose a serious human health, social and economic load. Aneuploidy-inducing agents in the cell target DNA, chromosomes, cell membrane, mitosis, and even cell receptor signaling pathway, and induce aneuploidy a variety of mechanisms, so that the environment-Africa aneuploidy inducers detect the presence of a variety of different means and terminal indicators, but are not perfect and comprehensive. In order to effectively detected in a variety of different mechanisms of aneuploidy inducers provide a scientific basis for the protection of human health, it is necessary environmental compounds Central Africa aneuploidy inducers or mitotic inhibitors investigate the mechanism. Ahead proven aneuploidy-inducing agents, cholinergic receptor antagonist the phosphatidylinositol pathway / inhibitor, from C - mitosis (C - M) effect analysis, nuclear / cytoplasmic configuration analysis, cell second messenger content changes in different angles, the ability to induce aneuploidy confirmed the test substance at the same time to explore their mechanism of action. Including: (1) C - M effect, dual-core cell frequency analysis, the end of the cell - the early G1 nuclei / cytoplasm split configuration analysis, start from the analysis of chromosome segregation to cytokinesis coordination, in vivo and from the circumstances of the two cholinergic receptor antagonist atropine (atropine, ATR), D-tubocurarine (d-tubocurarine, d-TUB) and KMSHT root aqueous extract (Tripterygium hypoglaucum ( level) Hutch, THH) three subjects Master of Yunnan Normal University graduate dissertation material ability to induce aneuploidy in mammalian somatic cells and mechanisms; (2) in vitro cultured Chinese hamster V 79 cells, at the same time Determination = $ base glycerol (iacylglycerol DAG) content and analysis of C-an M effect from chromosome segregation, the inositol phospholipid phthalate pathway angle, to further explore the known aneuploidy-inducing agents THH, colchicine (colchicine COL) benzene = phenols (hydroquinone, HQ) induced aneuploidy possible mechanism. The results showed that: (i) in mouse bone hate cells, toxic leather base type acetylcholine receptor (M receptor) antagonist ATR significantly induced C-M effect at the highest dose group (5.Zmglkg) (P <0.00 to 0.05); nicotinic acetylcholine receptor (receptor) orange Hang agent d-TUB in the low-dose group (0.063mg from g) only lead to more pronounced C- an increase in M ??cells (P <0.05); THH in the tested dose range p40-686 |