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Chrysotile Block Centriole Reposition during Cytokinesis Failure in HeLa Cells

Author: RenXiaoHui
Tutor: ShiQingHua
School: University of Science and Technology of China
Course: Genetics
Keywords: Chrysotile Binucleate cells Cytokinesis failure Of cytokinesis ditch degradation Centriole repositioning Living cells in real-time photography
CLC: R730.2
Type: Master's thesis
Year: 2011
Downloads: 4
Quote: 0
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Abstract


Chrysotile asbestos as a cause the strongly carcinogenic agent of malignant mesothelioma , while known . At the same time , binucleate cells induced by chrysotile asbestos is considered to be a transitional phase leading to aneuploidy and cell transformation . However , chrysotile cells induced by dual-core is still not known what internal mechanism . Previous studies have shown that , in the period of post-anaphase , centrioles to intermediates at repositioning during exercise , this process is essential for the completion of cytokinesis next . Processed chrysotile asbestos in order to study whether it would disrupt the a normal the centrioles campaign process , we use the real-time photography to track the movement of centrioles in HeLa cells induced by chrysotile dual-core process of living cells . In order to better display centrioles and nuclear material , in a targeted manner in HeLa cells stably expressing the green fluorescent protein (GFP) - labeled the centrin1 protein and red fluorescent protein (mCherry) labeled H2B histone . The results show that , as expected , the chrysotile asbestos treatment may be induced Among the different cell lines clear double nucleation effect , binucleated cells is usually generated by the degradation of the cytoplasmic bridges . Chrysotile treated cells , centrioles movement show a significant increase in the proportion of abnormal cells , and centrioles movement also delayed . More importantly , we observed that chrysotile treated cells , the centrioles the abnormal movement cytokinesis ditch degradation and has a close relationship between the dual-core technology . In summary , in the process of cytokinesis , normal centrosome intermediates repositioning can be blocked chrysotile treatment , perhaps this can be explained to some extent why chrysotile to induce dual-core effect .

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CLC: > Medicine, health > Oncology > General issues > Tumor pathology, etiology
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