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The Analyse of the Relation of Polymorphism of BoLA-DRB3 Gene and Subclinical Mastitis in Holstein Cows

Author: WangYanLiang
Tutor: HeGaoMing
School: Shihezi University
Course: Clinical Veterinary Medicine
Keywords: Mastitis Survey BoLA-DRB3 gene PCR-RFLP SCC
CLC: S858.23
Type: Master's thesis
Year: 2009
Downloads: 14
Quote: 0
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Abstract


Mastitis is a common disease of cows , is one of the most serious diseases caused by dairy industry losses . Cows MHC , also known as bovine lymphocyte antigen ( of BoLA ) , DRB3 gene is one of the most abundant polymorphism loci highly expression plays an extremely important role in the body's immune system in the animal , and many diseases of resistance , susceptibility there is a close relationship . Therefore, the the study cows BoLA-DRB3 polymorphism with recessive mastitis correlation of Subclinical mastitis prevention research is of great significance . Test a BMT the reagent mass of 150 cattle Shihezi subclinical mastitis survey . Survey of 574 cows , positive head count to 306 , the prevalence rate is about 53.31% , mammary positive rate was 24.15% . Test two selected subclinical mastitis and healthy cows in 150 groups , 80 in each , using the Hae III BstY I digested BoLA-DRB3 gene polymorphism and mastitis correlation analysis by PCR-RFLP method . The results are as follows : PCR amplification of a 305bp specific fragment ; restriction analysis of five alleles detected nine genotypes . The Hae Ⅲ restriction sites of base mutations deviation from Hardy-Weinberg equilibrium (P lt; 0.01), while BstYI restriction sites base mutation of the Hardy-Weinberg equilibrium state ( P gt ; 0.05) . The genotype Hae Ⅲ BB and genes of Hae Ⅲ B may be associated with susceptibility to mastitis related (P lt; 0.01); the genotype of Hae Ⅲ AA genes Hae Ⅲ A and mastitis resistance related ( P lt; 0.01 ) . Genotype and gene frequency of BstYI digested in infected cattle and healthy cattle distribution difference not significant with ( P gt ; 0.05 ) , it can not determine whether certain genes and genotypes with bovine mastitis resistance or susceptibility association. The the genotype of Hae Ⅲ BB and BstYIBB with high SCC related ( P lt; 0.01 ) , of Hae Ⅲ AA with low SCC related ( P lt; 0.05 ) .

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Livestock, poultry, wildlife diseases > Livestock > Cow
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