Dissertation > Excellent graduate degree dissertation topics show

Studies the effect of Nitric oxide on the course of Parasites infection

Author: FuXiaoPing
Tutor: ShenYongLin
School: Nanjing Agricultural College
Course: Preventive Veterinary Medicine
Keywords: Nitric oxide Fasciola hepatica Goat E. tenella Chicken
CLC: S852.723
Type: Master's thesis
Year: 2000
Downloads: 99
Quote: 2
Read: Download Dissertation

Abstract

After the test from the two aspects of the role of NO in parasitic infections: (1) goats infected with Fasciola hepatica (Fasciola hepatic), comparison of NO production between the amount of IgG antibodies between correlation; (2) chickens infected with E. tenella (Eemiria tenella), add nitric oxide precursors (L-arg) whether the protective effect of chicken. Goats infected with Fasciola hepatica, with nitric acid enzyme reduction Determination infection group goat serum nitric oxide (NO) levels (n = 5), found that one week after infection, the infected group decreased 2-6 weeks or high or low a wavy shape after six weeks has been higher than that in the control group (n = 4), which reached a peak 7-8 weeks (the 93.73 ± 17.233μmol / L). Indirect ELISA method the extracted with live adult excretory-secretory products ESAg established the earliest in the first two weeks after infection can be detected in the infected group antibodies (P <0.01), and maintain a high level from the first five weeks. Analysts believe the level of nitric oxide in the infected serum IgG antibodies correlation, the correlation coefficient of r = 0.511 (P <0.05); while the control group was only r = 0.25 (no difference), shows that the level of NO schistosomula transitional, adult parasitic. Infection eosinophilic leukocytes was significantly increased after infection 2-13 weeks, P <0.01, with NO changes of IgG; white blood cell count has been higher than that in the control group (no significant difference), red blood cell count in the first 7 weeks significantly decreased (P <0.01). The test results show that NO, IgG and EOS participatory role of host anti-Fasciola hepatica infection. NO detection method using the same, measured chickens infected with E. tenella plasma NO content increased significantly, especially in the six days after infection, reached the peak NO infectious dose and found no correlation. In the case of the same infectious dose, no significant differences in the the arginine group chicken plasma levels of NO and dexamethasone group, and in the infected control group without drug between, but this group significantly chicken weight, mortality, OPG and blood The dung number is low, the cecum gross and microscopic lesions were mild. Dexamethasone (iNOS inhibitor) chickens infected group (50ug / only) by the extent of the damage, the highest mortality, its plasma NO levels than non-infected control group significantly increased, presumably rupture cecal mucosa epithelium inflammatory cytokines activate iNOS, or dexamethasone insufficient inhibition of iNOS and other reasons related. The infection control group of indicators is somewhere between the two groups. The test results suggest that NO Eimeria tenella indirectly inhibit or kill role.

Related Dissertations

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Basic Veterinary Science > Livestock parasitology > Veterinary protozoan school > Cryptosporidium
© 2012 www.DissertationTopic.Net Mobile