|
Seabuckthorn oil is rich in nutrients, contain flavonoids, beta-carotene, a variety of vitamins, unsaturated fatty acids, amino acids and trace elements. Antioxidant role has long been subject to the attention of the majority of nutritionists and medical workers, and a lot of research work. Generally caused by the movement to generate a large number of free radicals in the body, they attack the cell membrane system, resulting in cell structure and function disorders. This study attempts to reveal the supplement Hippophae rhamnoides oil moving mice nutritional status, exercise capacity, free radical defense system, esterase isozyme and liver, cardiac and brain cell ultrastructure impact, to develop seabuckthorn genus as sports health food reference. 90 male ICR (Swiss Hauschka) mice, 7 weeks old, weighing 20 ~ 22g, purchased by the Experimental Animal Center of Shaanxi Provincial Academy of TCM, according to the experimental component caged purchase of the basal diet. The adaptive feeding week after experiment. The mice were randomly divided into a control group and the training group within the two groups are located Hippophae rhamnoides oil group and the control group. The mice Liberal intake of water and food, animal room at room temperature 22 ℃ ─ 27 ° C, humidity 40% ─ 65% of the lighting time along with the natural changes. The control group received the basal diet, Hippophae rhamnoides oil group the gavage Hippophae rhamnoides oil 0.08ml, basic forage. Hippophae rhamnoides oil by Shaanxi Hua late seabuckthorn Industrial Co., Ltd. seabuckthorn pharmaceutical production, the main ingredient, see 4.2.1. Training group six weeks of swimming training, water temperature 30 ± 2 ℃, the water depth of 35cm, swim 6d a week, every Monday, weighing four. The first weeks of daily swimming 30min, then 10min per week plus a day to five weeks swimming 65min sixth week of daily swim 75min. Prior to sacrifice, the last exhaustive swimming without load, exhaustive judgment standard: mice were submerged in the water for more than 10S, and can not be completed on the plane when the righting reflex. After exhaustive exercise training group was divided into recovery were sacrificed after 24 hours to recover for 24 hours immediately after exercise group and exercise group, and immediately after exercise group were sacrificed immediately after exhaustive exercise, after a 24-hour group exercise recovery exhaustive exercise. Each group were eyeball blood collection, preparation of serum measurements aspartate transaminase (GOT) and glutamic pyruvic transaminase (GPT), off marrow were sacrificed immediately after removing the brain. Liver, kidney, quadriceps muscle and heart, and placed in ice saline wash blood with a dry filter paper, and distilled Low homogenate was centrifuged (8,000 rpm, 15min), to take the supernatant was measured of SOD, MDA. At the same time, the rapid take groups into the heart, liver and brain tissues lmm3 about electron microscopy samples before fixed in 2.5% glutaraldehyde. Followed by 1% tetroxide hungry fixed in acetone for dehydration the Shaanxi Normal Institute of Physical Education, Human Movement Science Professional master's thesis agent, soaked in liquid for of propylene oxide EPON812 mixture; complete the embedding and polymerization with Swedish LKB -NOVA the thermal expansion ultramicrotome slice double staining stained with uranyl acetate and lead citrate, and finally with the Japanese production JEM-2000EX transmission electron microscope to observe and take pictures. The proper amount of the above-mentioned organizations, homogenate centrifuged, spotting, polyacrylamide gel electrophoresis, to study the impact of sea buckthorn oil and swimming training the esterase isoenzyme electrophoresis bands. The experimental results showed that: 1) seabuckthorn oil the oral solution mice appetite normal growth and development. 2) Training instantly within the group, the seabuckthorn oil oral solution group GPT, GOT was significantly lower than the control group. 3) SOD activity, quiet Hippophae rhamnoides oil group was significantly higher than that of the control group; immediately after exhaustive exercise Hippophae rhamnoides oil group was still significantly higher than the control group. Kidneys, myocardium, quadriceps and brain SOD activity training instantly Hippophae rhamnoides oil group was significantly higher than that of the control group. Group performance of the organizations of the majority of the content of MDA Hippophae rhamnoides oil group was significantly lower than the corresponding control group. 4) different tissue SOD activity, MDA content in the same state is not the same; same organization in different states, SOD activity, MDA content is not the same, which may reflect the differences in various tissues and organs antioxidant capacity. 5) Hippophae rhamnoides oil group, liver, kidneys, myocardium, quadriceps and brain tissue esterase isoenzyme electrophoresis band number and intensity of adaptive changes. 6) the structure of Hippophae rhamnoides oil group liver, cardiac muscle and brain tissue mitochondria, rough endoplasmic reticulum, myofilaments complete than the control group, the severity is lighter than the control group. The results of the experiment showed that: Hippophae rhamnoides oil can significantly improve the mice swimming ability, and significantly improve the movement of liver cells, myocardial damage some tissues of the body, but also improve the function of anti-free radical oxidation; synergy with endurance training role, can induce the liver, kidney, heart, quadriceps and brain tissue esterase isozymes adaptive changes occurred; also on the liver, heart and brain tissue ultrastructure has protective effects.
|