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Effect on Immunization of Mice or Cattle Against Inactivated E.coli (DH5a) Carrying Inhibin Plasmid pCIS

Author: FuPing
Tutor: YangLiGuo
School: Huazhong Agricultural University
Course: Animal Genetic Breeding and Reproduction
Keywords: Mice Cattle Inhibin pCIS Gene immunization E. coli Inactivated vaccine
CLC: S852.5
Type: Master's thesis
Year: 2007
Downloads: 10
Quote: 0
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Abstract


Application of the test gene immunization bacterial vector, enzyme-linked immunosorbent, B ultrasonic technology, research inhibin gene immunization of mice and cattle anti inhibition of antibody production, reproductive performance, security, inactivated E. coli passed inhibin gene vaccines may be a way to improve the method of inhibin gene immunization effect, will lay the foundation for the establishment of new technologies to improve animal fecundity. The main test are as follows: 1, Experimental study of the target gene of Escherichia coli containing the inactivated vaccine in mice in this experiment, the mice were divided into four major groups, the three experimental groups and two control groups. Each experimental group was divided into three different doses. The test group were injected: pCIS / procaine pCIS / DDA and inactivated bacteria; injected in control group: DcDNA3.1 (-) and PBS. The results show that: after the first immunization, the inhibition of E. coli treated antibody to P / N value and the average level of up to 2, no significant differences (P> 0.05) between the three doses; the procaine and DDA processing group 50μg dose group antibodies induced P / N value than the other two dose significantly (P <0.05); processing each group of E. coli P / N value to be lower than plasmid preferably the value of the dose group, but the difference was not significant (P > 0.05). Strengthen the immune, P / N value of each test group have increased, but there was no significant difference (P> 0.05), compared with control group a significant difference (P <0.01), and the other similar to the results of the initial immunization. After immunization induced antibody positive number of cattle, little difference between the E. coli treated with three doses, but the difference between the dose of plasmid group. Procaine group in the second highest after immunization antibody-positive cattle, 90%, and a minimum of 50%; smallest DDA group in strengthening the immune antibody positive rate of only 30%. The litter size, farrowing inactivated E. coli group compared with the control group, little difference between the dose; compared to plasmid group, the difference was not significant; maximum litter size 50μg dose of procaine group group. Maternal weight and organ index after immunization showed no abnormal situation. Overall, the inactivated vaccine immunized mice containing the gene of Escherichia coli results achieved with an adjuvant treatment difference is small, but the effective dose of inhibin gene vaccine that can reduce the immune injection, using E. coli to pass simultaneously immune effect is not reduced, and therefore to use E. coli to pass inhibin gene vaccine is feasible, can be promoted to large animals. 2, containing the target gene of Escherichia coli inactivated vaccine cattle Experimental research of this study, PMSG, the inhibin plasmid vaccine and carrying the target plasmid E. coli inactivated vaccine to immunize cattle. Bare plasmid immune adjuvant the procaine and DDA, three doses of inactivated vaccine component. The results showed that: P / N value of each experimental group after the initial immunization are relatively low, strengthen the immune P / N value of each experimental group was significantly higher, strengthen the immune detection antibody levels 20 days after each experimental group P / N values ??declined. Inactivated bacteria immune cattle, three different doses of the P / N value in the immunization are not significantly different (P> 0.05); differences between them, compared with the two plasmids immunohistochemistry not statistically significant (P> 0.05). Inhibin antibodies with PMSG treatment 10 head of cattle throughout the immunization period P / N values ??are lower. Procaine and DDA treatment groups after the first immunization antibody positive cattle are two inhibin antibody positive cattle to 3 after the second immunization; group of three different doses of the inactivated bacteria produced by the antibody-positive number of cattle not the same as the maximum dose twice immunity induced the inhibin antibody positive number of cattle is the most. Estrus cow ovaries detection results show that B-inactivated bacteria group number of mature follicles and adjuvant treatment of two plasmid group little difference between the number of mature follicles than PMSG treatment group development number was significantly different ( P <0.05). Aspects of ovulation, 10 head of cattle with PMSG treatment in six rows of dizygotic, four more than the control group; the inactivated bacteria treatment smallest dose group cattle three rows of multi-eggs, the greater the dose induced row dizygotic percentage greater. The negative cattle test group and positive cattle inactivated bacteria treated with the intermediate dose group antibody positive cattle mature follicular development the number of negative cattle compared to no significant difference, more than 0.92, the other two dose groups with antibody negative the mature follicular development number of cattle has a significant difference (P <0.05). The above analysis shows that with inactivated bacteria immune cattle the PMSG treated quite different, however, and the the plasmid group difference was not significant, because the maximum dose of the experimental design of three dose induced row dizygotic relatively good, so we can not determine the optimal dose of inactivated bacteria immune cattle, and cattle breeding rate the judge ultimately depends calving situation, so the study needs further study.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Basic Veterinary Science > Serological
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