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Death Signal"Transduction and Modulation Mediated by Fas on Fibroblasts Derived from Pathological Scars

Author: LuFeng
Tutor: GaoJianHua
School: First Military Medical University
Course: Plastic Surgery
Keywords: Keloid Gap junctions Fas Cell communication Ceramide Ca2 LPO H ~ PCR-SSCP Gene mutation
CLC: R62
Type: Master's thesis
Year: 2000
Downloads: 67
Quote: 0
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Abstract


BACKGROUND AND PURPOSE: Our previous experiments: FasMcAb not induce apoptosis keloid Fas receptor highly expressed in normal fibroblasts, while its intracellular and extracellular protein Bc1-2 positive expression rate is relatively low, suggesting derived suppressor: scar lump into the fiber of Fas-mediated death signal channel blockers. Clear keloid fibroblasts Fas-mediated death signal channel blockers reasons and specific blocking sites, we Fas-mediated disease scar fibroblast cell death signaling pathway study aims to explore the cause of keloids into the root cause of the abnormal fibers apoptosis. Method and content: using tissue culture techniques, surgical removal of keloids, hypertrophic scars and normal skin fibroblast tissue origin in vitro culture as an object of study; 1. Application adherent cell cytometry and comparing a variety of sources the cell gap junction-mediated intercellular communication. 2 to FasMcab for processing factors, to explore Ca 2 , H of the LPO (hydrogen peroxide) in the corresponding death signal channel. After detection and compare FasMcab of hypertrophic scars and keloids fibroblasts ceramide changes. 3 silver staining PCR-SSCP screening of Fas gene mutations, the last a suspicious positive PCR sample for DNA sequencing. . Results: 1 normal skin into fiber cells communicate with normal communication between hypertrophic scar fibroblasts cells suppressed keloids into fibroblast cell communication between blocked. 2. Under in FasMcab role, hypertrophic scars into the fiber of intracellular Ca 2 , LPO, ceramide was significantly higher and keloid fibroblasts Ca 2 , LPO, nerve amide unchanged; under in FasMcab role, hypertrophic scars, keloid fibroblasts H decreased between the two groups no significant differences. 3 detected by the PCR-SSCP, 20% (2/10) of the keloid tissue abnormal electrophoretic bands, suggesting that there may be Fas gene mutations. Two cases mutations are missing in the the Fas cDNA of 755bp at \Conclusion: cell communication proved with the contact inhibition of cell growth and invasive growth of cells is closely related to communication between cells is blocked through this experiment revealed was \biological mechanisms. 2 * as mediated apoptosis exception may be tumor keloid fibroblast proliferation - apoptosis abnormal regulation of cell biological mechanisms. FasM0 Hill. Under the action of the intracellular Ca2 +, LPO obstacles may be a direct result of abnormal sputum Keloid apoptosis. 3. As a second messenger in the Fas-mediated death signal channel, tumor keloid to produce defective the nerve Diaminodiphenol fibroblasts. Fas-mediated epilepsy keloid fibroblast death signal conduction block \#. . Encoding ras molecules death domain structural gene frameshift mutation may lead to the Fas receptor loss of function, leading to abnormal apoptosis of fibroblasts

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CLC: > Medicine, health > Surgery > Plastic Surgery (repair surgery )
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