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Umbrella tree (Acacia saligna (Labill.) HLWendl.) Mimosa Branch (Mimosaceae) gold acacia (Acacia) tree species, introduced into China from Africa and Ethiopia. The umbrella tree seeds, seedlings, leaves and stems, test materials, seed germination method and umbrella tree umbrella tree tissue culture of the two aspects of the problem, the main results are as follows: the umbrella tree seed germination study three different methods that concentrated sulfuric acid etching, ethanol soaking, hot water soaking the umbrella tree seed treatment germination test results show that the concentrated sulfuric acid etching, ethanol soaking, hot water soaking suction umbrella tree seeds inflation rate, germination potential, germination rate have varying degrees of impact. Hot water soaking the umbrella tree imbibed rate highest three treatment levels up to 100%. The germination rate is soaked in hot water 80 ℃ 40min for best germination rate of 90% germination potential of 72.15%. The anhydrous ethanol soaking the effect with 98% concentrated sulfuric acid etching effect is not obvious. Analysis of variance and multiple comparison showed that the hot water 80 ℃ soaking 40min with all other average germination rate of seeds treated with a very significant difference, is the best approach. The umbrella tree tissue culture research results show that: (1) For the umbrella tree stem segments, the best sterilization time to 75% alcohol 15s and 0.1% HgCl210min umbrella tree leaves, best sterilization time 75% alcohol 10s and 0.1% HgCl2 5 min (2) integrated umbrella tree callus callus rate, the callus growth screened MS basic medium for best callus induction. (3) umbrella tree leaves and stem segments with axillary induced callus, the leaves induced callus callus rate of 93.33%, higher than the stem segment callus rate of 81.25%, and the color of the callus texture are better than callus induced by stem segments, filter out the leaves to induce callus explants. (4) 2,4-D concentration of 1.0 mg / L and 2.0 mg / L, the callus out more highest rate of 100%, callus color, texture, and 2,4-D on umbrella tree callus inhibit the differentiation of multiple shoots, without using callus induction. (5) in a combination of 6-BA and NAA ,6-BA 2.0mg / L NAA 0.5 mg / L callus induction rate was the highest, up to 100%, so choose umbrella tree callus induction MS 6-BA 2.0 mg / L NAA 0.5 mg / L medium better. (6) in the axillary bud induction, MS induced axillary buds highest, up to 88.77%, for optimal induction of axillary bud germination medium. Germination effect of 6-BA on axillary best, ,6-BA0 1.4 mg / L NAA 0.2 mg / L for optimal induction of axillary bud germination hormone combination. The germination of axillary buds subculture in the culture of different concentrations of KT when KT is 0.5 mg / L, axillary bud elongation best results.
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