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ISSR-Assisted Research Conventional Cross Breeding of Pleurotus Eryngii

Author: LiuXiaoHong
Tutor: YeAiHuaï¼›CaiWeiMing
School: Anhui Agricultural University
Course: Botany
Keywords: Eryngii ISSR Variety comparison Single spore isolates Genetic hybridization
CLC: S646
Type: Master's thesis
Year: 2010
Downloads: 103
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Abstract


In this experiment, the experimental material eryngii ISSR molecular marker technology throughout eryngii traditional genetic crossbreeding process aimed bred good Pleurotus traits and molecular markers technology in the edible fungus traditional provide the basis of genetic crossbreeding. The experimental results are as follows: 1. ISSR (Inter-simplesequence repeat) molecular marker technology eryngii varieties from different regions of the country on 30 kinds of genetic diversity analysis. The study results show that 16 ISSR primers screened out bands of 202, 158 were polymorphic, multi-state rate of 78%, showing the rich ISSR genetic polymorphism; the use of NTYS-PC2. 1 software UPGMA analysis found that the genetic distance between 0.55-0.92, average genetic distance of 0.74 this 30 were divided into eight major categories of the experiment is fully illustrated China's eryngii varieties exist than large genetic differences, so as to filter out fine varieties of large genetic differences lay a good foundation. 8 class from this genetic difference the larger varieties selected from each Pleurotus test material, by Mycelia body growth, fruiting bodies of agronomic traits, yield and crude polysaccharide content and a series of biological characteristics and economics traits integrated filter out 2-3 quality Pleurotus. The results showed: X12, X29 and X30 traits best pro the best choice. Different mating type single spore separation and ISSR analysis of integrated traits elected three more excellent eryngii varieties The results show: the results of the results of the use of the traditional single-spore isolation method ISSR analysis method, four different mating type different mating types of the various varieties of different degree of variability. isolated traditional cross-breeding experiments, the hybrid offspring and their parents a preliminary comparison of the mycelial growth rate and ISSR Analysis of Genetic Diversity. The experiments show that: hybrids and their parents showed good genetic diversity, the genetic distance between 0.58-0.88, and X29 and X30 and X12 and X30 hybrids mycelial growth rate than pro the better performance , X12 and X29 hybrid offspring appeared partly degraded. In summary, this experiment has not only been 14 hybrids, to lay the foundation for further identification and selection of superior strains, also to understand the whole process of growth and development as well as edible fungi ISSR polymorphism analysis in edible fungi The edible fungus crossbreeding to provide technical support.

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CLC: > Agricultural Sciences > Gardening > Vegetable gardening > Fungi ( mushroom )
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