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In order to study CO 2 in tissue culture role in the process, to explore the process of tissue culture-free (at least) the possibility of sugar cultivation, designed and produced CO 2 Real-time monitoring systems. The system consists of incubator, computer systems, monitoring equipment and supply components in four parts, and designed a monitoring software. The system can be set using the culture environment CO 2 concentrations within the set range, can grow in tissue culture sugar, CO 2 enrichment, high photosynthetic photon streams and sterile environment. In this paper, traditional tissue culture environment CO 2 concentrations were determined to simulate its curve of the day, found that the traditional way of tissue culture plantlets cultivated a strong photosynthesis ability, in the light shortly after (within one hour), a small bottle CO 2 concentration decreased rapidly to the compensation point, after the period in tissue culture in the entire illumination CO 2 \In the lateral illumination of 65μmol · m -2 · s -1 (photosynthetic photon flux density, PPFD), the top light is 12μmol · m -2 · s -1 , the bottom of the light of 43μmol · m -2 · s -1 , photoperiod of 12 hours / day, temperature under the condition of 25 ℃, use CO 2 real-time monitoring system for grape (\1500ppm) of CO 2 , with the traditional tissue culture methods for a period of 20 to 30 days of the three comparative experiments, each culture methods are divided into sugar (10g / l) and sugar groups. Experimental results show that through the system by Shi CO 2 for photoautotrophic (sugar culture, group 1) and photosynthetic and dependents (with sugar cultivation, group 2) grape seedlings, leaf area, the average growth rates were 90.0% and 140.9%, stem diameter growth rates were 76.6% and 79.7%, branches high growth rates were 62.8% and 44.2%; conventional tissue culture methods, the sugar culture (group 3) and with sugar training (group 4) leaf area average growth rates were 53.8% and 197.1%, stem diameter, the average growth rates of 1.6% and 39.1%, branches high growth rates were 30.2% and 100.3%. Group 2, the average dry weight is 1.7 times the group 1, group 3, 6.6 times, 2.8 times in group 4. This suggests that enriching CO 2 plantlets cultivated grapes, especially those with sugar cultivation, with a more pronounced beneficial domestication traits: body stout stems, festival short pitch, the root system, dry matter heavy accumulation of many. Meanwhile, slice the stem microscopic observation showed that enriching CO 2 plantlets bred significantly advance the overall development. The results show that: the CO 2 real-time monitoring system to achieve the sugar culture plantlets (photoautotrophic), by increasing the applied CO 2 for no (low) sugar Tissue culture than the traditional way has obvious advantages.
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