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Anthurium (Anthurium andraeanum Lind.) is among the most popular ornamental plant of Araceae, with beautiful showy flowers, exotic foliage. Anthurium, using bulking up via a callus stage, followed by adventitious bud formation, has been proposed as an interesting possibility for large scale production. Nowadays, most Anthurium cultivars were propagated by ways of tissue culture. Light and gas environment around the plantlets were very important in plant tissue culture. In this study, the factors affecting in vitro propagation of A. andraeanum were studied, and besides studied the in vitro growth and photoautotrophic capability of A. andraeanum by using light emitting diode (LED) and sugar-free culture. The results are as follows:1. A protocol for callus inducton and plant regeneration of A. andraeanum Lind in vitro plants has been successfully developed. Leaf sections were used as explants, the results showed that the content of NH4NO3 reduced to 1/4 in the MS medium, the frequency of leaf callus was significantly enhanced. When the content of TDZ was 0.4 mg·L-1, the leaf had better ability to form callus. The frequency of callus formation was highest on modified MS supplemented with TDZ 0.4 mg·L-1 and 2,4-D 1.0mg·L-1. The optimal medium for callus differentiation was 1/2 MS+BA 0.2 mg·L-1+KT 0.5mg·L-1. The half strength MS supplemented with BA 0.2mg·L-1 and KT 0.5mg·L-1 was suitable to plantlet proliferation and the combinations of BA 0.2 mg·L-1 and KT 0.5 mg·L-1 added into IBA 0.2 mg·L-1 was suitable to plantlet growth.2. By using light emitting diode (LED) to accurately modulate spectral energy distribution, this paper studied the effects of light quality on tissue culture and growth of A. andraeanum. Red LED was the most effective for callus induction from leaf explants. Compared with dark, light treatments were suitable for adventitious shoot formation, and under red LED and fluorescent, the frequency of adventitious shoot formation from callus piece was the highest. Plantlets under red light exhibited the highest soluble sugar content and stomata frequency. However, monochromic blue LED induced abnormal growth. The maximal leaf area, root length, fresh and dry mass, and soluble sugar content were obtained with red+blue LED. Compared with fluorescent light, red+blue LED showed the obvious advantages on fresh and dry mass, pigment contents and carbohydrate contents, and was propitious to breed vigorous plantlets and decrease energy consumption.3. By using light emitting diode (LED) to accurately modulate spectral energy distribution, and with fluorescent light as the control, this paper studied the effects of light quality on the growth and photosynthesis of transplanting plantlet of A. andraeanum. Under red+blue LED, the leaf area, root length, and root vigor were significantly higher than those of other treatments, and the soluble sugar content was significantly increased as well. Under blue LED, the chlorophyll and carotenoid, starch and protein content reached the maximal and were significantly higher than those under red LED. Values of Fv/Fm,ΦPSⅡ, ETR were higher under blue LED, red+blue LED and fluorescent light than those under other treatments. Under red+blue LED, the net photosynthetic rate was significantly increased.4. The effects of CO2 enrichment on growth and development of A. andraeanum under red+blue LED were investigated using sugar-free culture. The results showed that the plant height, leaf area, root vigour, fresh and dry mass of leaf and root, content of soluble sugar and protein of A. andraeanum were significantly increased under 1000μmol·mol-1 CO2 after 30 day’s culture compared with the common culture condition. It indicated that CO2 enrichment for A. andraeanum plantlets would promote the growth and photoautotrophic capability of the plantlets, and also would short the multiplication cycle.5. The effects of photoperiods on the growth and photoautotrophic capability of A. andraeanum in sugar-free culture under red+blue LED were investigated using auto-control system of gaseous microenviroment for plant tissue culture. The results showed that the plant height, leaf area, fresh and dry weight, soluble sugar contents, net photosynthetic rate and values of Fv/Fm, Fv/Fo,ΦPSⅡ, qP of 14 h·d-1 treatments were significantly higher than those of 12 h·d-1 treatments, but there was no significant between 14 h·d-1 treatments and 16 h-d"1 treatments. It indicated that the growth of plantlets in sugar-free culture of A. andraeanum was promoted and the photoautotrophic capability of plantlets could be improved by 14 h·d-1 treatments.
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