Dissertation > Excellent graduate degree dissertation topics show

The Research of Tissue Culture in Fast Breeding Technology for Osmanthus Fragrans Lour

Author: LiangMaoChang
Tutor: LiuYouQuan
School: Central South University of Forestry Science and Technology
Course: Tree Genetics and Breeding
Keywords: Osmanthus Fragrans Lour stem embryo leaf tissue culture
CLC: S685.13
Type: Master's thesis
Year: 2008
Downloads: 129
Quote: 0
Read: Download Dissertation


The experiment purpose is to do the research of Osmanthus fragrans Lour. plant tissue culture which happened in variety of ways. The experiment contained two facets of tissue culture research.One facet is that taking the young tress,germfree seedling stem and embryo as explants to regenerate in the form of germfree short tress cutting.The other is that taking young leaf,embryo as explants to occur in the form of callus.The results are as follows:1 Tissue culture research of regenerating in the form of germfree short tress cutting(1)Germfree system established The seed which inoculated at low contaminat-ion rate is easy to disinfect. Using Hgcl20.1% to disinfect young new tress for 3-6 minutes is available.The top bud is at low contamination rate.(2)Initial culture comparing with medium B5 and WPM, medium MS is more available for embryo initial germinating;Less than the dosage of 1×1014 N+·cm-2 is available for embryo germinating when N+ immitting in the embryo body.Dealing with the seed in the environment of low temperature 4℃and cold storage for 210 days can break embryo dormancy in effect,accelerating the embryo to bourgeon in advance. Comparing with medium B5 and MS, medium WPM is more available for young stem germinating;While controlling the stem not to be browned,the AC concentration should be controled within 0.1%.(3) Multiplication culture for embryo plants B5+0.50 mg·L-1TDZ+0.05 mg·L-1 NAA is available for tuft buds inducement. B5+5.00 mg·L-1VBA+0.10 mg·L-1NAA, B5+1.00 mg·L-16-BA+1.00 mg·L-1GA and WPM+0.50 mg·L-1 TDZ+0.05 mg·L-1 NAA+0.50 mg·L-1BR are available for elongation and multiplication culture of embryo plants.The plant with ion beam implantation in embryo is at about 2.04 cm high in elongating culture process; The plant with no ion beam implantation in embryo is at about 2.77 cm high in elongating culture process; The diversity between each other is notable(4) Multiplication culture for stem plants B5+7.00 mg·-16-BA+0.10 mg·L-1 NAA,B5+0.10 mg·L-1TDZ+0.05 mg·L-1NAA are available for tuft buds inducement. B5+8.00 mg·L-1KT+0.05 mg·L-1NAA,B5+2.00 mg·L-16-BA+1.00 mg·L-1GA,B5+2.00 mg·L-16-BA+0.10 mg·L-1BR,WPM+0.50 mg·L-1TDZ+0.05 mg·L-1NAA+0.50 mg·L-1 BR and B5+2.00 mg·L-6-BA+3.00 mg·L-1Biotin are available for stem elongation inducement.(5)Rooting culture 1/2MS+0.60 mg·L-16-BA+4.00 mg·L-1 NAA is available for rooting culture inducement.2 Tissue culture research of occuring in the form of callus(1)Initial callus inducement culture WPM+0.12 mg·L-12,4-D is available for embryo callus inducement. B5+0.12 mg·L-12,4-D+1.50 mg·L-16-BA is available for young leaf callus inducement. B5+5.00 mg·L-16-BA+0.10 mg·L-1NAA is available for young sterile plant leaf callus inducement.(2)Multiplication callus inducement culture WPM+0.50 mg·L-1TDZ is available for embryo callus multiplication. WPM+0.10 mg·L-1BR+0.10 mg·L-1 NAA is available for leaf and embryo callus multiplication.(3) Polarization callus inducement culture B5+0.40 mg·L-1TDZ+0.10 mg·L-1 +0.50mg·L-1BR is available for embryo callus polarization. WPM+1.00 mg·L-1TDZ +0.05 mg·L-1NAA and B5+5.00 mg·L-16-BA+0.10 mg·L-1NAA are available for leaf callus polarization.(4)Rooting culture 1/2MS+0.60 mg·L-16-BA+4.00 mg·L-1NAA is available for rooting culture inducement.

Related Dissertations

  1. Numerical Simulation and Contrastive Analysis of Labyrinth Seals and Leaf Seals,TK263.2
  2. Effects of Light Control on Tissue Culture and Photosynthesis Characterristics of Anthurium Andraeanum Lind,S682.14
  3. Study of Intracerebral Transplantation of Umbilical Cord Blood Mesenchymal Stem Cells in a 6-Hydropamine Rat Model of Pakinson’s Disease,R742.5
  4. Screen and Analysis of Specific Expression Gene Promoter in Stem and Leaf of Rice,S511
  5. Study on Rapid Propagation of Energy Plant Euphorbia Tirucalli,Q949.93
  6. Monitoring of Insecticide Resistance and Resistance Risk Assessment for JS118 in the Rice Stem Borer, Chilo Suppressalis (Walker),S435.112.1
  7. Detection of the Resistance in Xanthomonas Oryzae PV. Oryzae and X. Oryzae PV. Oryzicola to Two Bactericides and the Assentment of Resistance Risk in Vitro,S435.111.4
  8. Effect of Planting Densities and Modes on Lodging Resistance of Stem in Summer Maize,S513
  9. The Comprehensive Evaluation of Flue-cured Tobacco Origin Place Environment and Tobacco Leaf of Fangxian,S572
  10. Effects of the Nitrogen Management and Interactions with Leaf Number on Growth Yield and Quality of Burley Tobacco,S572
  11. Study on the Key Techniche of Tissue Culture of New Phalaenopis ’EVER Greatduble Dragon,S682.31
  12. Studies on Leaf Epidermal and Pollen Micro-morphology and Geographical Distribution of the Genus Euonymus of Henan,Q944
  13. Study on Sorghum Sudanense Leaf Spot Caused by Bipolaris Sorghicola,S435.4
  14. Studies on Senescence Difference in Roots and Leaves in Two Varieties of Maize and Their Regulator,S513
  15. The Difference of Proteome Expression of Low Light Stress and Regular Light Recovery on Growth of Maize Seedling,S513
  16. Primary Study of Inheritance on Lodging Resistance Traits in Maize,S513
  17. Monitoring and Managing for Fungicide Resistance in Sclerotinia Sclerotiorum,S435.654
  18. Screening and Evaluation of Antagonistic Organisms Against Pyricularia Grisea and Xanthomonas Oryzae PV.Oryzae,S435.111.4
  19. Study on the Recovery of Growth and Its Physiological Mechanisim of Cotton After Waterlogging During the Bud Stage and Flowering and Boll-Forming Stage,S562
  20. Effects of Leaf Trait and Water-Fertilization Status on the Contents of Main Volatile Aroma Components in Flue-cured Tobacco of Central Henan,S572
  21. QTL Analysis for Yield in a Japonica Rice (Oryza Sativa L) RIL Population,S511.22

CLC: > Agricultural Sciences > Gardening > Ornamental Horticulture ( flowers and ornamental trees) > Flower trees in class > Osmanthus
© 2012 www.DissertationTopic.Net  Mobile