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The plant will be subjected to many environmental factors, the limiting factor, low temperature stress is one of the most important abiotic stress factor, low temperature a serious impact on plant growth and development and geographic distribution. With the study of plant cold physiological mechanisms and molecular mechanisms of the deepening and biotechnology are maturing, through genetic engineering techniques and tools provides a new way for the cultivation of the cold New Varieties of Plants. In recent years, proteomics methods and techniques applied to the study of plants, and achieved certain results. Proteomics method of plants to low temperature stress response mechanism, to explore low adversities associated protein, and then explore the molecular mechanism of plant hardiness provide important theoretical basis, in order to improve the resistance of plants temperature. In this study, the warm season turfgrasses, 1 floor (of Buchloe dactyloide of (Nutt.) Engelm.cv Zhongping No.1) bison grass for material, buffalo grass seedling leaves under low temperature stress cell membrane permeability and protection activity changes; using two-dimensional electrophoresis, mass spectrometry combined with bioinformatics, buffalo grass in 5 ° C low temperature stress 0h, 6h and 24h differential proteomics research. Bison grass physiological and biochemical changes under cold stress, Differential proteomic expression, as well as through the low temperature molecular mechanisms of bison grass low temperature stress response protein identification, to explore the buffalo grass cultivated for the future cold and strong buffalo grass new varieties has laid a theoretical foundation to provide a theoretical guidance for the production of practical activities in response to chilling injury. The results of this study are as follows: a certain limit stress on the cell membrane permeability of the buffalo grass blades will not cause permanent damage. Low temperature stress on the cell membrane causing some damage, the greater the stress the longer the cell membrane damage taken, but under the stress of a certain limit, the cell membrane does not cause permanent damage. 2 buffalo grass seedling leaves under low temperature stress defense enzymes inconsistent trend. With the stress time, the leaves of SOD, POD and CAT activity showed variation for the first and then decreased, but the changes in the three enzymes differ, POD activity performance is volatile, when the stress continued until 24 h, POD activity was significantly lower almost flat compared with the control, the more sensitive the POD affected by low temperature; SOD activity has maintained a high level; activity of CAT and SOD activity consistent, showing the adjustment process of these two enzymes at low adaptability in synergy. 3 bison grass seedlings under low temperature stress analysis of two-dimensional gel electrophoresis. The Trizol precipitation Preparation of protein samples, using the classical two-dimensional gel electrophoresis protein separation buffalo grass seedling leaves, high resolution and reproducible dimensional electrophoresis profiles, at least 850 protein spots detected on the electrophoresis pattern. After ImageMaster 2D Platinum V 5.0 software, at a certain point in time, changes in the expression abundance of significant differences in protein spots of 87, including 60 upregulated and downregulated 27. Part 4 of differentially expressed proteins identified by mass spectrometry and functional analysis. 26 relative abundance of high protein selected from the 87 differentially expressed protein spots in the MALDI-TOF-MS analysis, 17 protein spots were identified, including known and new low-temperature stress response protein . These proteins are involved in the following metabolic under chilling stress in buffalo grass procedure: the metabolic pathway of carbohydrate metabolism, energy metabolism, cell signaling, sulfur and fatty acid metabolism, protein processing, and cellular defense. Combined with other plants that have been reported in 17 kinds of protein function, a preliminary study of the low-temperature-resistant mechanism of bison grass. There is also an unnamed protein and hypothetical protein, these two proteins function in plant adaptation to low temperature stress regulation mechanism needs further study.
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