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Flow injection chemiluminescence method in drug analysis and environmental analysis

Author: YangJiDong
Tutor: HuangYuMing；LiuShaoZuo
School: Southwest China Normal
Course: Analytical Chemistry
Keywords: Chemiluminescence Drug analysis Flow Injection Environmental analysis Promethazine hydrochloride Applied Research CL spectra The head hold Qusum sodium Fluorescence spectrophotometer Chlorpromazine hydrochloride
CLC: O657.3
Type: Master's thesis
Year: 2001
Downloads: 167
Quote: 1
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Abstract


Chemiluminescence (Chemiluminescenc, referred to as CL) is the chemical reaction of the reactants or products to absorb the chemical reaction releases energy electronic transitions from the ground state to an excited state, and then return to the ground state from the excited state generated by light radiation. This method has the advantages of high sensitivity, wide linear range, the equipment is simple, rapid and easy to automate. Has broad application prospects of the trace analysis as an effective method in the field of environmental science and life sciences. This paper studies the Ce (IV) - promethazine hydrochloride system, K 3 [Fe (CN) 6 ]-luminol-phenazine drug system, Ce (IV) - cephalosporin class of beta-lactam antibiotics-Rh6G system, NBS (N-bromosuccinimide) - hydrazine - luciferase system, NBS-ammonium - fluorescein system chemiluminescence behavior, influencing factors, analysis chemical properties and environmental analysis and drug analysis. 1.CE (IV) - of Promethazine Hydrochloride system in 0.015 mol / L sulfuric acid, sulfuric acid, cerium oxide hydrochloride promethazine produce chemiluminescence, a detailed study of the impact factors of the reaction, under optimum conditions isoproterenol hydrochloride The triazine measured linear range of 1.0 × 10 -7 g / mL ~ 1.5 × 10 -5 g / mL, the detection limit is 4.0 × 10 -8 g / mL, 5.0 × 10 -6 g / mL promethazine hydrochloride measured 11 times, and the RSD was 2.6%. This Act in hydrochloric acid analysis promethazine tablets, compared with the Pharmacopoeia, with satisfactory results. 2.K 3 [Fe (CN) 6 ]-luminol-phenothiazine drugs system in strong alkaline conditions, the two thiophene thiazide drug hydrochloride Exclusive promazine and chlorpromazine hydrochloride the K 3 Fe (CN) 6 -lu system of light-emitting strongly enhanced fluorescence spectrophotometer were measured by the following system chemiluminescence spectrum: 1) luminol potassium ferricyanide NaOH; 2) the luminol iron potassium cyanide NaOH promethazine hydrochloride; 3) the luminol iron potassium cyanide NaOH chlorpromazine hydrochloride. The results showed that the two drugs studied only from the sensitizing effect of Luminol - potassium ferricyanide-NaOH system, because there is no new luminous body produce. Under optimum conditions, the CL intensity within a certain range of these drugs is directly proportional to the concentration, method with high sensitivity, detection limit of promethazine hydrochloride 3.0ng/mL, hydrochloride summary chloropropyl choke The detection limit is 5.0 UN puzzle L, the method used for the determination of the drug in tablets with satisfactory results. 3. CE p-head holding sand meat to anti-the master Li · n6G system in addition to hydrolysis under strong alkaline conditions, the hydrolysis in acid, alkali or neutral conditions, including phenol has a sulfur-containing amine antibiotics last Jibu SH) of the compound generated, the study found that the Ce (IV) oxide the SH) sparse Jibu-containing compound can produce weak luminescence N6G can greatly enhance this weak luminescence, thereby establishing a measuring head hold cephradine, cephalosporin spicy, cephalosporin macrophage fatty sodium, the cephalosporin ceftriaxone sodium cephalosporin throat ketone sodium cephalosporin class, including phthalocyanine amine antibiotics FIA-CL analysis. Fluorescence spectrophotometer were measured the following chemiluminescence spectrum; l Chuan * / L sulfuric acid steel +5 X10 'ffiol few system with rhodanmine 6G +0 .Zffiol/LHZSO4: 2) 10 nind / L sulfuric acid persimmon + SX10 id few n6G +0.2 i * / L HZSO4 +30 hg / L of the head to hold the system ketone degradation product; 3 Nakagawa n * a few sulfuric acid iridium + SX10 \showed that the decorative oxide N6G sulfuric acid under acidic conditions to produce weak chemiluminescence, but the degradation products after adding two head holding a strong emission peak appears in the vicinity of 560 urn, where the peak position corresponds exactly to the N6G fluorescence peak. under the best conditions, has been tested and 5 heads hold antibiotics concentration 1 knife X108g/ffiLI.SX10 a g / hl range chemiluminescence intensity showed a good linear relationship. 5 knife X10'g / iL head hold the sample n repeated measurements, the relative standard deviation (RSD) head hold pull head bubble Weixin, head hold spit fat, sodium cephalosporin sodium Qusum, the cephalosporin send ketone sodium were 2.20,2.50 , 2.7O, 2.8o, 2.80. detection limit mortar a) head lamp pull set, the cephalosporin spicy, the head hold the dry moat Li Na head hold Qusum sodium, head to hold it ketone sodium the knife X 10 'eggs L, of 3.SX 108 g / mL, 3.8 XI 08 g / ml, 3.9 XI 08 g / ml, 4 of knife XI 08 g / IL 4 was XI 08 g / mL. ScS preparation of common coexisting substances and is used to hold the head of the synthesized samples analysis results satisfactory 4.NBS-speak - the fluorescein system in the knife 5 mol / L of alkaline conditions, NBS fluorescent pigment The sensitizing effect of oxidation sulfuric acid threats, strong chemiluminescence. Based on this paper a detailed study of the characteristics and influencing factors of the reaction, the threat of sulfuric acid under optimum conditions determination range of 0 leaves the knife X 10 \The detection limit is 3. OX 10 \with satisfactory results. 2 Abstract 5.NBS buildings - Fluorescent give system under the knife SM alkaline conditions, NBS fluorescein sensitizing effect oxidative chlorination, produce strong

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CLC: > Mathematical sciences and chemical > Chemistry > Analytical Chemistry > Instrument analysis ( physics and physical chemistry ) > Photochemical analysis ( spectral analysis method)
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