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Self- incompatibility is rapeseed heterosis utilization is one important way , but its molecular mechanism is rarely reported . In this study, Brassica napus self -incompatibility Department of S - 1300 and 124 pro and Brassica napus materials, clone S -1300 S-locus genes SRK , SP11 and SLG (BnSRK-S-1300, BnSP11- S - 1300 and BnSLG - S - 1300 ) SCAR markers can identify Brassica napus S-locus genotype system, SRK , SP11 expression in the S - 1300 , paternal and F 1 differences preliminary study Brassica napus self-incompatibility and molecular mechanism . Main results are as follows : 1 to obtain a S- 1300 SRK , SP11 and SLG full-length sequence of : BnSRK - S- 1300 for the 8367 bp in size , including the seven exons and six introns ; BnSP11 - S - 1300 Size 607 bp , contains two exons and one intron ; BnSLG - S - 1300 full-length gene sequence to 1672 bp in size , contains two exons and one intron . The sequence of the 2.BnSRK - S - 1300 and BrSRK - 60 the highest similarity of 98% ; concentration of sequence differences between them on the Exon4, Intron4, Intron5 and Intron6 ??. Compared with the BrSRK - 60 BnSRK - S- 1300 exon4 only 91 % sequence similarity , Intron4 Intron5 and Intron6 ??sequence similarity is not higher than 82%. BnSP11 - S - 1300 with Similar BrSP11 - 60 , up to 100 % , with BoSP11 - 15 only 97 % similarity , and has a 5 bp insertion and 2 bp deletion . BnSLG - S - 1300 with the highest similarity BrSLG - 60 , up to 99% , 98 % similarity with BoSLGB . Described above S - 1300 is a new S haplotype , and with the the Cabbage S - 60 may have a common ancestor . Established a SCAR molecular marker system , including two multiplex PCR mark (ISLG SP110, ISLGb-L SP11a) and a single PCR marker ( SRKa ) , can be used for identification of Brassica napus S-locus genotype . As the male parent , S - 1300 as the female parent and their the F of 1 < / sub > offspring for expression of molecular markers to select E ??genotype and genotype F Brassica napus . Northern blot and Realtime PCR results consistently show that , SP11 only in self- expressed incompatibility Department S - 1300 , S - 1300 , paternal and F 1 , SRK expressed in the female parent S - 1300 , the highest expression level .
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