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Gentian leaf blight the gentian disease, the occurrence of common, high incidence of economic losses. For gentian blight is still in the blank stage. This is the first clear gentian leaf blight pathogen, gentian seed carrier inspection and processing technology, the prevalence of disease and prediction system, from the seed treatment, seed processing technology, agricultural control measures, chemical pesticides to control, effective prevention and treatment of gentian leaf blight technology system to filter out the low toxicity and efficient chemicals, chemical control gentian active ingredient content, control techniques to improve the yield and quality of the herbs gentian, standardization of production laid the foundation for gentian Chinese herbal medicines. The first symptoms, the pathogenic form and inoculation determine the cause of gentian leaf blight pathogen Alternaria fungus Alternaria sp. In this study, the occurrence of disease patterns and forecast a mathematical model, disease index based on the different times of the year t y use CurveExpert software to find the best mathematical simulation model can reflect the relationship between the two - logistic model and changes in condition and environment (temperature, humidity, rainfall) factor combined application software to establish the condition and temperature, rainfall, humidity regression equation: the Y 1 =-in 0.150X 1 1.436X 2 -0.855X 3 -6.843 (R 1 = 0.8603, R 2 = 0.4528, R 3 = 0.2544) Y 2 =-0.126X 1 1.325X 2 -0.832X 3 -15.263 (R 1 = 0.7689, R 2 = 0.5163, R 3 = 0.1956) (Y 1 < / sub> for the three-year-old gentian disease index; the Y 2 for the the biennial gentian condition index; X 1 is the temperature; X 2 humidity; the X 3 rainfall), can be used as a model of forecasts. Seed treatment technology study found that pharmaceutical treatment to the applied music 500 times Polyoxin and 300 times, a prop 300 times, 1,000 times the world of high, mancozeb 5000 times can completely kill the pathogen, and the germination rate did not much impact. And pharmaceutical processing the verification after the contamination, again identified by the above-mentioned pharmaceutical antibacterial effect. In this experiment, the agricultural control measures of gentian leaf blight control effect, different spacing corn intercropping on both sides of the gentian plot found to 50cm intercropping density control effect best, the control efficiency of 51% for the prevention gentian leaf blight effective agricultural measures. Seedlings pharmaceutical treatment against gentian leaf blight results show that different drug-treated seedlings have a preventive effect, Shi Squeak 400 times the best control effect reached 79.8%, followed by the world of high efficacy was 70.1%, the worst for a prop, the control efficiency of 44.1%. 20 fungicides Efficacy test, established virulence regression equation, determination of the EC50 and EC100. Found that the pharmaceutical gentian leaf blight bacteria have a significant inhibitory effect, the strongest inhibition rate of 25% applied to one hundred grams of gentian leaf blight pathogen EC50 for 0.92μg/mL, EC100 10μg/mL. Followed by 10% of the world's high-EC50 1.78μg/mL, EC100 200μg/mL A 70% of care is the worst EC50 5623.41μg/mL. Field efficacy trials confirmed inventory of 1000 times the effect of the best anti-efficiency up to 63.7%, followed by the World 1000 times higher, the control efficiency of 62.2%. Determination of the content of the active ingredient in the gentian herbs according to the results of the field trials, further evaluation of the screening of fungicides on the quality of gentian. Glycosides to gentiopicroside and swertiamarin indicators, use of chemical pesticides to control gentian leaf blight, it will affect the active ingredient content of gentian, relative to the control group will increase the content of active ingredients, such as gold mine, the World Higher fungicides, and some will be a slight decrease, such as Amistar.
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