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Gentian leaf blight disease gentian, common, high incidence of large losses. In this study, based on previous studies, research gentian seed carrier inspection and processing technology, disease epidemics and prediction from the seed treatment methods, agricultural control measures, seed processing, chemical pesticide control methods and Chinese medicine to prevent methods point of view, the establishment of effective prevention and treatment measures gentian leaf blight, part of the determination of pesticide residues and pesticide residue limits, and provides a basis for the pesticide safe medication gentian in planting. The experiment for the occurrence of disease patterns and forecast a mathematical model, disease index based on the different times of the year t y use CurveExpert software to find the best mathematical simulation model can reflect the relationship between the two logistic model, condition seasons change and the environment (temperature, humidity, rainfall) factor binding, the application software to establish the condition and temperature, rainfall, humidity regression equation: Y =-0.387X1 2.381X2 for-1.461X3-6.261 (Y biennial disease index, X1 humidity temperature, X2, X3 rainfall); Y-=-0.324X1 1.486X2-3.228X3-6.439 can be used as a forecasting model. (Y for three years disease index, X1 temperature humidity, X2, X3 for rainfall). Research seed infection detection technology and processing technology found that seed testing is the most practical, the most convenient way in a petri dish germination test; hot water treatment to 50 ° C, 15 min and 60 ° C, 5 minutes gentian seed germination relative smaller, gentian seeds can kill pathogens, three kinds of traditional Chinese medicine ethanol extract of gentian seed treatment results showed that the three Chinese herb extracts when more than 30%, the inhibitory effect can reach 90%. Traditional Chinese medicine preparation process gentian seed security, environmental characteristics, can be used as the gentian deal with new environmentally friendly fungicides. In this experiment, the agricultural control measures gentian leaf blight control effect, gentian on both sides of the furrow spacing corn intercropping 50cm control efficiency up to 51.1%, for the prevention and treatment of gentian leaf blight effective agricultural measures. 20 fungicides Efficacy test, established virulence regression equation, determination of the EC50 and EC100, the pharmaceutical gentian spot blight have significant inhibitory effect, 10% of the world of high gentian leaves strongest blight pathogen inhibition rate for 0.97μg/ml, EC100 62.5% microg / ml, followed by more than 3% of antimycin, 68% of the gold mine, 25% of the inventory, 40% pyrimethanil. The gentian field test choose better agent in the indoor efficacy experiments. The seed processing test results show that to pyrimethanil 800 times the best results, the control effect of 70.3%, followed by Stroby 800 times, 400 times mancozeb, thiophanate-methyl, 600 times, 1000 times the world of high The effect is also good. The seedlings more concentrated, easy to handle, can receive better control effect. Field applied pesticides to control gentian leaf blight results show that the inventory of 1000 times the best control effect, followed by the gold mine 400 times, the pyrimethanil 1000 times, the World 1000 times the effect good. Better chemical control C propiconazole, inventory for pesticide residues dynamic analysis. The study is the first use of propiconazole, pyrimethanil control of Radix Gentianae diseases, pesticide residues in herbs dynamic sampling and analysis at different times after treatment, pesticide residues dynamic equation, the development of the highest pesticide limits and the safe use of standard, the establishment of the dragon The gall propiconazole residues in gas measurement method and gentian Pyrimethanil liquid method. Therefore, propiconazole residues, does not affect the quality of the herbs gentian as the prevention and treatment of gentian leaf blight effective agents. Pyrimethanil medication for 22 days, the residues still higher than the MRL value, extend the interval of the proposed use.
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