Dissertation > Excellent graduate degree dissertation topics show

Studies on Meat Quality Through The Method of Candidate Gene in Chicken

Author: WangQiGui
Tutor: LiHui；LiNing
School: Northeast Agricultural University
Course: Animal genetics and breeding
Keywords: F2 chickens Meat quality traits Extracellular fatty acid binding protein gene Myogenin gene The mononuclear past acid polymorphism
CLC: S831.2
Type: Master's thesis
Year: 2001
Downloads: 278
Quote: 22
Read: Download Dissertation

Abstract


Of poultry breeding practices of the past few decades considerable progress has been made. Chicken daily gain, feed conversion ratio and its resistance has been greatly improved, the choice of such high intensity, however, brought the negative impact of the succulent flavor decline. These problems has caused breeders around the world attach great importance to and has made some achievements, but on broiler subcutaneous fat and abdominal fat content is too high, partial bottom of the chest and thigh intramuscular fat content improved little progress. Intramuscular fat (intramuscular fat, IMF) and meat quality and taste was positively related to affect meat tenderness, flavor and juiciness, tenderness of the meat. Depth understanding of the the chicken body adipose tissue development and growth mechanism is of great significance to improve the content of intramuscular fat, control of excess fat deposition (abdomen, around the crop with the multitude of fat), and improve the quality of chicken. In this study, the star broilers and Silky hybrid the F 2 on behalf of the experimental material, PCR amplification with 12 pairs of primers for chicken extracellular fatty acid binding protein gene 5 'regulatory region and coding Four pairs of primers EPO gene promoter region of the chicken muscle cells for PCR amplification and SSCP analysis of amplified PCR fragment after polymorphism fragments were sequenced. Chicken extracellular fatty acid binding protein gene amplification part of a total of eight point mutations, -1807 at AG-1805C-A, -1011 TC, -1000 at the insertion of the C 659 TC 924 CT 968 CA -TG 1985 CT mutation. Myogenin promoter region of the gene were found in three point mutations were -618 TC -224 GA and -160 C base insertion. The of various genotypes chicken chicken extracellular fatty acid binding protein gene carcass traits for statistical analysis, the results derived extracellular fatty acid binding protein gene second pair of primers genotype BB and AB and AA genotype higher abdominal fat weight and abdominal fat percentage, the ninth primer pair HH genotype compared with other genotypes have lower abdominal fat weight and percentage of abdominal fat, the differences were highly significant (P <0.001 ), and other carcass traits (carcass weight, breast muscle weight, leg muscle weight, liver weight) there is no significant difference (P> 0.05) between these genotypes. Therefore speculate that the fragment as a marker of fat choice does not affect other carcass traits. We can draw the following conclusions, chicken extracellular fatty acid binding protein gene may be a major gene traits in chicken fat or major genes controlling the traits chain, and the invention of DNA markers that can be used on chicken fat traits for molecular marker-assisted selection.

Related Dissertations

  1. Studies on CDC16 Gene, EFNB2 Gene Polymorphisms and Relations with Partial Economic Traits in Pigs,S828
  2. Effects of Feeding Model on Welfare of Broiler,S831
  3. Molecular Characterization, SNP Detection and Their Asscociation with Meat Quality Traits of Bovine MYPN and ACOX1 Gene,S823
  4. The Distribution and Polymorphisms of CHEMERIN and Its Receptor CMKLR1 Gene in Qinchuan Cattle,S823
  5. Studies on ADIPOR1 and CTSF Genes Polymorphisms with Their Relations to Partial Economic Traits in Pigs,S828
  6. Studies on Effects CCKAR Gene and Its Association with Partial Economic Traits in Pigs,S828
  7. Germplasm Characteristics of Nuogu Pigs,S828
  8. Cloning, SNP Dection of Porcine CACNA1S Gene and Association with Meat Quality Traits,S828
  9. Dietary forage ratio Holstein young bulls on growth and meat quality,S823.5
  10. Study on the Polymorphisms in Calsarcin-1, GHRH Gene and Their Relationship with Growth and Carcass Traits in Four Chinese Indigenous Yellow Cattle,S823
  11. Studies on SNPs of DECR1 Gene and Its Relations with Partial Economic Traits in Pigs,S828
  12. Study on the Polymorphisms of CAPN1 Gene and Their Relationships with Meat Quality Traits in Yak,S823.85
  13. The Study of Crossing Effect of Princess and Huaixiang Chickens and Analysis of the Molecular Genetic Characteristics of Ex-FABP Gene,S831.2
  14. Nanyang cattle μ-CAPN polymorphism Correlation Analysis with Meat Quality Traits,S823
  15. Study on Meat Qualities of FI Hybrid with Boar and Laiwu Gigs,S828
  16. Effects of Chronic Heat Exposure on Fat Deposition and Fat Metabolism in Male and Female Beijing-You Chickens,S831
  17. Selection of the Main Candidate Genes Which Affect Meat Traits of Mongolia Bovine,S823
  18. Investigation of Molecular Markers Linked to Meat Quality Characters of Chicken,S831.2
  19. Studies on SNPs of DGAT1 Gene and Its Association with Partial Economic Traits in Pigs,S828
  20. Studies on Effects of AMPD1 and MYF6 Genes Polymorphisms on Partial Economic Traits in Pigs,S828
  21. The Correlation Analysis among the Microsatellite DNA Markers and Pit-1 Gene for Meat Quality Traits and Carcass Trait in Hybrid Pigs,S828

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Poultry > Chicken > Genetics, Breeding
© 2012 www.DissertationTopic.Net  Mobile