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Antisense RNA transcription vector construction and shrimp transgenic technology research
Author: LinXiongZuo
Tutor: YangFeng
School: Third Institute of Oceanography of State Oceanic Administration
Course: Marine biology
Keywords: actin promoter shrimp transgenic anti-virus
CLC: Q782
Type: Master's thesis
Year: 2001
Downloads: 205
Quote: 0
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Abstract
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It is widely accepted that disease caused by WSBV (white spot bacilliforni virus) is responsible for the heavy economic losses cast on prawn culture. Currently, there are no effective chemicals or antibiotics to treat viral diseases. Vaccination also cannot yet be used to prevent disease in shrimp because, unlike vertebrates, shrimps lack a memory immune response based on antibody production. It is plausible to develop an anti-virus prawn strain drawing supports from the anti-sense and transgenic technique, The construction of an anti-sense vector is involved in the strategy for the new anti-virus-prawn strain developing. So the goal of our work is to obtain the actin promoter in shrimp, which is essential for the construction of the transgenic vector in shrimp transgenic field. Four kinds of actin promoter and three kinds of actin terminator were cloned and sequenced based on an improved adaptor ligation method. Moreover, DNA polymerase gene was identified in the WSBV genome, which provide us a good target gene in the shrimp anti-virus transgenic Research. Once transcript the anti-sense RNA of the target gene, the transgenic shrimp will acquire the capability of anti-WSBV through blocking the activity of the enzyme in RNA level. At last the anti-sense RNA transcriptional vector was constructed and used in Transgenic Research, and the medium and parameters of electroporation transgenic were confirmed. all the work will achieve the future succeed in the shrimp transgenic anti-virus research field.
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CLC: > Biological Sciences > Molecular Biology > Genetic engineering (genetic engineering) > Gene vector
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