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ANTIGENIN, DYNAMIC OF LHRH ANTIBODY AND SECURITY OF GENETIC ENGENIEERING LHRH VACCINE

Author: GongGuangMing
Tutor: JiChuanYi；LuChengPing
School: Nanjing Agricultural College
Course: Preventive Veterinary Medicine
Keywords: Luteinizing hormone- releasing hormone LHRH Lactation type luteinizing hormone- releasing hormone mLHRH The avian type luteinizing hormone- releasing hormone aLHRH Trx thioredoxin Carrier protein Trx-mLHRH L1 Trx-mLHRH-aLHRH-mLHRH L2 Antigenic cross-cutting Antibody Dynamic Immunopotentiating Safety Evaluation Antigen residues Amino acid sequence analysis
CLC: S852.4
Type: Master's thesis
Year: 2001
Downloads: 94
Quote: 1
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Abstract


Indirect ELISA crossover trial and cross-inhibition experiments prove the avian type LHRH nursing LHRH between the antigenic cross very weak to make genetic engineering LHRH the castration vaccines livestock dual-use, must be the avian type LHRH nursing LHRH tandem to achieve The intended purpose. LHRH antibodies detected in mice two weeks after a free low antibody titers in the eighth week, two free antibodies peaked three to four weeks after the seventh week, the eighth week of antibodies decreased rapidly. Antibody Dynamic test pigs free pigs in a second week of production of antibodies, antibodies in the detection of a critical level in the seventh week, two free antibody titer slightly more than one exemption, continued until the eleventh week low titer , a Free. Weak antigens reinforcing rabbit intradermal test, three states antigen vaccine produced antibodies in the test time are high level maintained, and a small change in LHRH antibody production in mice injected test larger changes, but rabbits and The mice tests were remodeling group that Staphylococcus aureus the LHRH vaccine immunogenicity in immune complex group; both stronger than single seedlings group. Castration vaccine rabbit residue experiment, the rabbit blood, muscles 2 days, 4 days after the liver, spleen after 10 days in ELISA sensitivity range is not detected the residue of LHRH antigen. The immune rabbit mice fed the test showed that consumption of potentially harmful reproductive health immune castration animal meat. Immune castration, surgical castration is not castration control group, three groups of pork quality inspection, immunological castration can remove the meal taste in nutrients immune castration and surgical castration was no significant difference, but once immunohistochemistry pig testicular Size no difference with the control group, swine testicular slight atrophy secondary immunization group. Rabbit hyperimmune serum passive immunization preliminarily confirmed edible immune castrated animal meat (even raw), reproductive health will not be affected because the antibody anti-hormone effect. The fusion protein amino acid sequence analysis of the existing LHRH, did not find any existing protein sequence thereto highly homologous, homology mainly concentrated in the carrier (thioredoxin protein) portion, the tertiary structure analog to obtain a carrier thioredoxin protein three-dimensional structure, relatively LHRH antigen hydrophilic and hydrophobic properties of the two fusion proteins, indicating that breast-feeding type of LHRH in the antigenicity and lactation-type LHRH avian type LHRH in the L1 and L2 corresponding to some of the same amino acid hydrophilic hydrophobic may be different.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Basic Veterinary Science > Animal Immunology
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