Dissertation > Excellent graduate degree dissertation topics show

The Study on Endothelin and Nitric Oxide iNOS mRNA Expression Changes in Site of Vein Transfer with Delayed Breaking Pedicle in Rabbits

Author: WangLiHua
Tutor: ZhengShuSen；PanSongLing
School: Zhejiang University
Course:
Keywords: Auto-vascular　transplantation Endothelin Nitric oxide Delay Nitric oxide synthase gene
CLC: R654.4
Type: Master's thesis
Year: 2001
Downloads: 34
Quote: 0
Read: Download Dissertation

Abstract


Nowadays auto-vascular transplantation is used widespreadly. Compared with artifical vascular, it has its superiority , but its late stenosis rate is still about 30-40% and attracts some vascular specialist?attention. In vascular surgery, how to prevent the transplanted blood vessel from stenosis has become the focus of research. It has been confirmed since 1987 that NO was a gas endogenous molecule of biological message. It was named “yearly molecule”by science magazine in 1992. NO is related to the tone of peripheral blood vessel, it also can diastole vessel, inhibit the excretion of VSMCs and its migration, and prevent monocyte and platelets from adhering to vascular endothelial cells. In vivi, NO is produced by NOS which can catalyze 5 L-Arginie. Usually NOS was divided into two subtypes, ie, cNOS and iNOS. Among them, the expression of cNOS mRNA is dependent on Ca2+ and Calmodulin (CaM), its concerntration is low stable within blood and tissue fluid. The transcription of INOS is by blood flow shearing force and induced by inflammatory factor such as TNF-α. IL-1. The expression of iNOS increases and maintains at a high level for a long time at the action of inducing factors. Some reports have showed that ET is an endogenous molecule which is antagonistic agent against the action of NO. Our study is to investigate the time at which the isolated rabbit femoral veins were transplanted, and NO within the transplanted vein increased and maintained at a high level for long time, which would hinder the hyperplasia of transplanted vascular intima maximally. Serium NO concerntration was measured by Griess method. First nitrate reductase reduced NO3 to NO2 , then NO2 reacted with Griess agent. The value of OD was representive of the level of NO. The method we used is more precise than others. 6 We evaluated ET concemtration by RIA and measured 1NOS mRNA by RT-PCR. Experimental results showed the best time for auto-vascular transplantation. It may provide theoretical evidence to clinical treatment in future. 1. Experimental material: Fifteen male Newzealand rabbits with thirty hind legs. Serium sample are from femoral vein (FV) of rabbit’s hind legs. 2. Experimental group and establishment of animal model. Fifteen rabbits were divided into five groups, each had three rabbits (six lengs). Group A: auto-vascular transplanted instantaneously Group B: auto-vascular transplanted with 2-hour-delayed. Group C: auto-vascular transplanted with 4-hour-delayed. Group D: auto-vascular transplanted with 8-hour-delayed. Group E: auto-vascular transplanted with 24-hour-delayed. Each group included three rabbits with six hind legs. 2.1 Auto-vascular transplanted instantaneously (group A) 7 Blood taken from FV after isolating femoral artery (FA) and femoral vein, and cut off them, then FV-FA interspersedly transplantation was performed immediately. 2.2 Auto-vascular transplanted with 2-hour-delayed (grou

Related Dissertations

  1. Modeling Internet Link Delay Based on Measurement,TP393.4
  2. Robust Model Predictive Control of Networked Systems,TP273
  3. Study on Relationship between Endothelin-1, Insulin Resistance and Acute Ischemic Cerebrovascular Disease,R743.3
  4. Studies on the Effects of Exogenous Nitric Oxide on Rice under Cadmium Stress,S511
  5. Tissue-engineered Construction and Preliminary Implantation Study of Diamond-like Carbon Films for Artificial Mechanical Valve Applications,R654.2
  6. Kinase Activities Analysis and Localization of ZmCCaMK Induced by Abscisic Acid in Leaves of Maize Plants,S513
  7. The Correlation Study between Entothelin-1 Level in Blood Plasma and Hemorrheology Changes in Primary Glaucoma and Visual Function Damage Degree.,R775
  8. Changes and Roles of Plasma Hydrogen Sulfide in Elderly Patients with Ischemic Heart Failure,R541.6
  9. The Relationship between Exhale Nitric Oxide and Serum Eosinophil Cationic Protein, IL-13, IL-8 in Acute Asthma Exacerbation,R562.25
  10. The Relationship of Serum Adiponectin、Nitric Oxide Synthase and Carotid Atherosclerosis in Patients with Acute Cerebral Infarction,R743.33
  11. Spectral Method for Solving Two Types of Delay Differential Equations,O241.8
  12. The Mechanisms of Propofol-induced Relaxation on Isolated Thoracic Aorta Rings in Rats,R614
  13. In Vitro Study of Theeffects of Fullerol on the Hippocampal Synaptic Plastisity,R741
  14. Oxidation Removal of Sulfur Dioxide and Nitric Oxide by Ozone in the Flue Gas,X701.3
  15. The Research on Data Fusion Algorithm and Model for Wireless Sensor Networks,TN929.5
  16. Angiogenic and Neuroprotected Roles of Erythropoietin Posttreatment on Neurovascular Unit in Oxygen Glucose Deprivation Injured Model: An in Vitro Study,R743.3
  17. Research on the Quality of Service Technology in IP Network,TN915.09
  18. Research on the IP QoS Technology Based on Diffserv Model,TN915.09
  19. Experimental Study of No_X Storage/reduction (NSR) Using DME as Reducer,TK46
  20. Ignition Delay Characteristics Study of Kerosene and Contaminated Kerosene in Shock Tube,TK16
  21. Study of Digital Substation Communication Simulation and Network Reliability,TM76

CLC: > Medicine, health > Surgery > Of surgery > Cardiovascular and lymphatic system surgery > Peripheral vascular
© 2012 www.DissertationTopic.Net  Mobile