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Explore the Function of Immunoregulation Network in Patients with Chronic Hepatitis B Virus and Chronic Hepatitis B Virus and Chronic Hepatitis C Virus and Their Differences

Author: DuanGuoRong
Tutor: ZhouYongXing
School: Fourth Military Medical University
Course: Internal Medicine ( Infectious Diseases )
Keywords: Viral hepatitis, hepatitis B, hepatitis C , pathogenesis, immune regulation, peripheral blood mononuclear cells , interleukin , interferon , T lymphocyte subsets Cytokines
CLC: R512.62
Type: Master's thesis
Year: 2001
Downloads: 77
Quote: 0
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Abstract


By chronic hepatitis B (CHB), the level of detection of cytokines in peripheral blood of patients with chronic hepatitis C (CHC), T lymphocytes film IFN-gamma, IL-6 expression and T lymphocyte subsets observed, from the cellular and molecular The two research CHB and CHC patients with immune regulatory function of changes in the CHB, CHC difference. First, the use of a double antibody enzyme analysis detected 35 cases of CHC, 30 CHB patients serum IFN-gamma, IL-2, IL-4, IL-6 and IL-12 level. The results showed that: CHB group Th1 cytokines IL-2 increased Th2 cytokines IL-4, IL-6 level increased, with the normal group than the P-value <0.05, significant difference. CHC group Th1 cytokines IL-2 increased Th2 cytokines IL-4, IL-6 level increased, with the normal group than the P-value <0.05, there are significant differences. CHB, CHC two groups of Th1 cytokines IFN-gamma and APC secretion of IL-12, compared with control group there were no differences (P> 0.05). CHB group Th1/Th2 ratio and the CHC group of Th1/Th2 ratio decreased, compared with control group P-value <0.05, significant difference. CHB group compared to the CHC group, no significant difference between the various cytokines. Disease group showed a Th2 type cytokine levels higher than the Th1 cytokines. Th2 cytokines levels increased and the resulting ratio of Th1/Th2 decline may be continued with CHB, CHC course. Second, the use of flow cytometry 41 CHB patients, 36 cases of CHC patients with peripheral blood T lymphocyte subsets. The results show: the the CHB group of peripheral blood of patients with CD 4 cells was significantly reduced, CD 4 / CD 8 ratio decreased significantly, with the control group than the P-value <0.01, significant difference, the CD 8 cells number was not significant, with the control group than P> 0.05, not statistically significant. The the CHC group of patients with CD 4 the cells decreased CD 8 cell count increased, CD 4 / CD 8 ratio decreased with the control group than the P-value <0.01, there are significant differences. CHC group CD 4 cells decreased the CD 4 / the CD 8 ratio decreased more significantly, the CD 8 cell count increased significantly, with CHB group than the P-value <0.05, significant difference. Fourth Military Medical University, a master's degree thesis experiments confirmed CHB, CHC patients do exist in vivo immune disorders. And prompts CHC patients with CD. \Cell count increased compared to the CHB group significantly hepatitis C (HC) where reason than chronic hepatitis B (HB) high. Isolated patients and normal peripheral blood mononuclear cells (PBMC) using anti-human T lymphocyte monoclonal antibody indirectly rabbits infected Fluorescence no stimulus conditions T lymphocyte membrane IFN-Y, IL-6 expression was observed and found that: The the the CHB groups and the CHC group IL.6 expression strong normal group (P <0 Knife 01); disease group between IL-6 expression difference (P> 0 knife 5); IFN-Y expression in the disease group are less than, more obvious to the CHC group, but no statistical difference (NO knife 5) reported in the literature Th class of cells to clear the virus infection plays an important role in IFN-Y, whereby prompt, CHB and CHC patients Thl cytokine IFN-Y of expression is low, may affect the clearance of the virus with the chronicity of the disease. In summary, 1.HBV, and HCV infection diffuse can lead to poor servant class cytokine secretion; Thl / ThZ ratio decreased. the 2.CHB and CHC patients not only serum cytokines produce proportional disorders was also found that peripheral blood T lymphoid Pat cell membrane is N-Y expression is also inadequate, patients with CHC is more obvious. CHB and CHC patients servant, THZ cells produce cytokines like volume, flow cytometry, however, found that patients with CHC CD. \+ / CD. Ratio decreased compared with CHB obvious, it may be (l) HCV low concentration in the blood of rabbit immunogenic immunostimulating weak; (2) PBMC HCV violations, IL12 low, results where low proliferation, IFN-Y inadequate secretion, HC slow Shi rate higher.

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CLC: > Medicine, health > Internal Medicine > Infectious disease > Viral infections > Viral Hepatitis > Hepatitis B
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