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The purpose of to observe severe traumatic shock in rats serum inflammatory cytokines IL-6, TNF-α and intestinal mucosal S-IgA phase change to provide a theoretical basis for the treatment of shock. By trauma, bled manufacturing rats with traumatic hemorrhagic shock model. 64 12-week-old SD rats were randomly divided into group A (normal group), B group (shock group) and group C (recovery group). Group B in accordance with the shock 30min, 60min, divided into three subgroups of B-Ⅰ, B-Ⅱ, B-Ⅲ 90min the resuscitation group in accordance with successful resuscitation 0h, 1h, 2h, 4h divided into C-I, C - Ⅱ, C-Ⅲ, C-Ⅳ four sub-groups of eight animals. Recovery group: Ringer liquid through the femoral vein to put blood volume of blood drawn with equal amount of reinfusion recovery within 30 min infusion rate of 1.0 ~ 1.5ml/min, blood pressure returned to the bloodletting before 90% or more successful resuscitation. The control group only underwent carotid vein and femoral vein catheterization, phlebotomy not only drawn; Other groups in each experimental phase after a severe traumatic shock and recovery experiments are based. Using a double antibody sandwich enzyme-linked immunosorbent assay (ELISA) determination of IL-6, TNF-α, the content of the intestinal mucus S-IgA, immunohistochemical detection of intestinal villi S-IgA expression. HE staining was used to observe the morphological changes of the gastrointestinal villi. All data by SPSS 16.0 software processing, the statistical results to the mean ± standard deviation (? X ± S), the groups were compared using independent samples t-test analysis. Results of a rat traumatic hemorrhagic shock, TNF-α in shock 30min portal vein and the inferior vena cava levels were not significantly elevated shock the 60min back door vein TNF-a began to increase, with the control group, a highly significant difference (P lt; 0.01), TNF-a shock 90min inferior vena cava was significantly higher, and the control group were significantly different (P lt; 0.05), peak recovery after 1h, with the control group, there is a very obvious difference (P lt; 0.01 ) 2h of recovery began a slow decline, the portal vein is elevated prior to the inferior vena cava, inferior vena cava elevated level above the portal vein. IL-6 before shock 90min compared with the control group, no significantly elevated, there was no significant difference (P gt; 0.05); significantly higher recovery 0h, there is a significant difference (P lt; 0.05) and the control group, after IL- 6 levels continued to rise, peaked recovery 2h, very significant differences (P lt; 0.01) with the control group, after slowly decline, its content is still significantly higher than that in the recovery 4h, there are significant differences (P lt; 0.05), the inferior vena venous blood content than the door venous blood, and trends. 2 small intestinal S-IgA-positive cells showed cytoplasmic staining shades of brown particles, their number and dyed shades represent the expression levels of high and low. IgA plasma cells in B-Ⅰ group begin with significantly reduced, and the time delay and decreased gradually until successful resuscitation after 4h began to rise, but compared with the control group, there was still a significant difference (P lt; 0.05) . Mucosal S-IgA is primarily located in the mucosal lamina propria. S-IgA content in the B-Ⅱ group began to significantly reduce time delay gradually reduced, compared with the control group, until 4h after successful resuscitation Although only a slow recovery, but still a significant difference (P lt; 0.05). IgA plasma cells with S-IgA content compared to the former lower earlier and faster, both successful resuscitation after four hours before beginning a slow rebound in. The gastrointestinal mucosa pathological findings, as the shock deepened gradually villous edema, shedding and necrosis, 2h from the the individual intestinal mucosa that repair phenomenon after successful resuscitation 4h part of the intestinal villi repaired, lamina propria fibrous tissue hyperplasia. Severe trauma - hemorrhagic shock rat model of an Institute of replication of the conclusion with the reproducibility, controllability, reliability, stability requirements, the copy is successful. 2 intestinal S-IgA content with the shock of prolonged decline, even if timely corrective shock, only to slow recovery trend in recovery after 4h. 3 intestinal IgA plasma cells, S-IgA and TNF-a, IL-6 content of the change was negatively correlated with the intestinal IgA plasma cells, S-IgA content decreased serum TNF-A, IL-6, the content increased, thus proving that TNF-a, IL-6 endotoxin and bacterial translocation of proinflammatory cytokines. TNF-a, IL-6 and S-IgA interaction, a vicious circle, which leads to further decline in cytokine \
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