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Objective: To observe the plateau, the plains environment eye contusion hindbrain myoglobin (neuroglobin, NGB), and transforming growth factor-β 2 (TGF-β 2 ) expression and its relationship with relations plateau contusion ocular injury and repair tissue hypoxia, aims to provide the basis for the treatment of eye disease in the case of clinical hypoxia. Methods: 50 rabbits were randomly divided into plateau contusion group, plateau contusion group, plain contusion group, plains contusion group. Plateaus, plains contusion group 3 rabbits (n = 12 eyes), the plateau contusion group of 21 rabbits (n = 36 eyes), plain contusion group of 21 rabbits (n = 36 eyes), respectively, making eye blunt contusion model (both eyes do), and the other: plain contusion 1d, 3d each increase of one rabbit, ready-line electron microscopy morphological observation. Eye contusion model preparation: after anesthetic ketamine 25mg/kg intramuscularly, its head placed in the the vertical fixed PVC pipe, steel ball (0.33kg) since the tube to the upper end of the vertical drop (height 1m) to the free fall way each hit once in the middle of the double cornea, causing his eyes light contusion model. Groups respectively after injury 2h, 6h, 1d, 3d, 7d, 10d, 14d observe corneal and retinal and immediately in the corresponding time points in the rabbit ear vein bolus of air, the animals were sacrificed after the immediate removal of the eye (in the last rectus attached marker at 12 o'clock the sclera shallow hanging line), respectively, along the isolated eye angle the 5mm ring after limbal incision, drawing to select the area of ??the cornea and retina edema turbid, immersed in 10% formalin fixative , soak 48h, embedded in paraffin. 6 spare, each rabbit eye producer for histological examination (HE staining) were measured by immunohistochemistry. Results: Immunohistochemical staining slice staining intensity of the positive area of ??quantitative analysis, positive staining positive area expressed the strength of the gray value Grey was negatively correlated with Grey value greater positive expression weak. Xylene were mounted, light microscopy, NGB protein immunoreactivity (NGB-immunoreactive NGB-IR) positive in the cytoplasm of brown particles appeared pale background. And determination of gray values, averaged eight randomly selected image scanning analysis of computer vision applications. Immunohistochemistry product dyed deeper, gray scale value is smaller, the higher the content of the antigenic material. Retinal ganglion cell layer in normal circumstances NGB-IR ganglion cells were strongly positive, positive the kernel layer neural cells NGB-IR, cone rod layer NGB-IR was weakly positive, but the pigment epithelium, outer nuclear layer, external network layer and the inner plexiform layer NGB-IR negative. The test model group NGB immunohistochemical staining positive retinal outer plexiform layer, inner nuclear layer, inner plexiform layer, ganglion cell layer and inner limiting membrane (see figure 3); NBG gray values ??were compared: plain group with consistent trend of the dynamic changes of the the plateau group NGB immune reaction product (NGB-IR) (see Figure 1), the increase in the 2h to 3d within four points with time, reached a peak in the 3d; gradually fell 7d But below the a 2h level of. Plains group the plateau group NGB retina expression time in seven points higher than non contusion group (P <0.05); plateau the group expression higher than the plain group, 2h significantly different (P <0.01, see figure 4. 5), but the plain group a higher increase. TGF-β 2 protein immune response (of of TGF-β 2 -immuno-reactive, TGF-β 2 -IR) positive corneal epithelial the cytoplasm of the cell layer, the stroma and the endothelial cell layer (see Figure 6), contusion group, the Plateau group with the plain group group compared the differences were statistically significant at each time point, the the plateau group with the plain group phase compare 6h, 1d, 3d difference significant (P <0.05, see figure 7,8), a different point in time dynamic curves show TGF-β 2 contusion early corneal expression over time increased late expression decreased, the plateau group was lower than the plain group (see figure 2), and lower expression in non contusion group, and no time-dependent. Conclusion: NGB expression in the rabbit retina organizations altitude hypoxia environment than the expression of NGB in the the rabbit retina organizations plains environment. Altitude hypoxia environment rabbit eye contusion the NGB of expression in the retinal tissue above the plains environment rabbit eye contusion NGB expression in retinal tissue. Altitude hypoxia environment NGB expression in retinal tissue growth is lower than in the rabbit eye contusion the NGB of expression in the retinal tissue of the rabbit eye contusion in the plains environment of growth. Rabbit eye contusion. Altitude hypoxia environment of TGF-β in the corneal tissue below the plains environment corneal damage TGF-β 2 expression 2 expression . Rabbit eye contusion mitochondrial structure in retinal tissue damage, degeneration.
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