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The background and purpose of deafness affect the quality of human life, and one of the major problems lead to permanent disability. Estimated number of global hearing-impaired about 278 million people, of which 2/3 in the developing world. Hearing damage not only to patients with a tremendous impact, but has become a serious social problems, the adult hearing damage is more than 50% of the world's top ten one of the leading cause of disability 65 years of age or older have a hearing impairment. China as a developing country, and with the social and economic development, the aging of society is more and more serious. Age-related hearing loss is associated with the aging of the auditory system (generally occurs in more than 60 years of age), occurs degeneration cause deafness. The reason ear microvascular degradation, the cochlear lesions as well as the whole body function degradation closely related. The presbycusis ear microvascular degeneration, which may be related to poor and ear microvascular injury repair and regeneration ability. Endothelial progenitor cells (endothelial progenitor cells, EPCs) play an important role in the repair and regeneration of the vascular injury. EPCs express a variety of endothelial markers and can be integrated into the new blood vessels in the ischemic region. Studies have shown that certain physiological conditions, a variety of factors such as illness and drug can endothelial progenitor cell mobilization, migration, settlement, integration and differentiation of functions to produce a clear impact. The body's aging, smoking and other unhealthy habits, lack of sports, myocardial infarction, hypertension, angina pectoris, cerebral infarction, Ⅰ and type 2 diabetes can lead to EPCs decrease in the number of functional decline. Endothelial progenitor cell function in patients with presbycusis how to change, and reports currently also uncommon. The EPCs function EPCs function in the peripheral blood of patients with presbycusis study with age-matched healthy older adults in the peripheral blood of contrast, to investigate the the EPCs function changes of patients with presbycusis, and further explore presbycusis The possible mechanism. Materials and Methods. Patient data and packet presbycusis patients 16 cases, including 7 women and 9 males, age 60-69 years, mean 64.7 years. Found the deaf history 9 months to March 2, the classification for patients with moderate deafness, speech frequency hearing threshold at 41 ~~ 55dB. Exclude a history of drug use may be an impact on EPCs and other recent medical history. The control group of healthy elderly volunteers were 16 cases, including 7 women and 9 males, aged 60-68 years old, with an average of 65.5 years old. Endothelial progenitor cell separation, culture take the object of study in peripheral venous blood fasting 10 ml of employing lymphocyte separation medium density gradient centrifugation, mononuclear cells, cultured in M199 medium (containing 20% ??fetal bovine serum and growth factor). 7 days of culture of multi-wavelength laser confocal microscope identification of FITC-UEA-I and Dil-acLDL double positive cells are differentiated EPCs. Progenitor cell colony and cell counts, the adhesion, proliferation, migration ability to detect the first 7 days to count groups EPC colony and cell number, then digested, collecting part of the culture of EPCs were the following experiment. Determination of adhesion: The cell seeding density of 1 × 105 / ml, and inoculated into a pre fiber connection protein (1ng/μ1) coated culture plates cultured 30min, counting adherent cells. Determination of proliferation capacity: cell density of 2 × 103 / ml, using the MTT cell proliferation and cytotoxicity kit EPCs proliferation ability was measured. Migration ability: a cell density of 2 × 104 / ml, using a modified Boyden chamber and cultured for 24 hours, counting the number of cells migrated to the lower chamber. Results in peripheral blood of patients with presbycusis endothelial progenitor cells in vitro EPCs colony count of 1.40 ± 0.37; the EPCs count of 54.32 ± 9.24; adherent cell count was 17.32 ± 5.18; EPCs absorbance of 0.154 ± 0.042; Boyden chamber migration to the underlying EPCs The number of 11.18 ± 2.73. Control group, peripheral blood endothelial progenitor cells cultured in vitro EPCs colony count was 2.88 ± 0.48; the EPCs count of 67.64 ± 8.39; the the adhesion cell count was 22.58 ± 5.46; EPCs absorbance of 0.226 ± 0.574; Boyden chamber migration to the underlying EPCs 15.72 ± 3.43. EPCs of the presbycusis patients with group the colony cell count, adhesion ability, proliferative capacity, migration decreased compared with the control group, with significant statistical difference (P lt; 0.05). Conclusions Elderly patients with deafness endothelial progenitor cells cultured in vitro cell count, adhesion, proliferation, migration decreased ability; endothelial progenitor cell function decline may be one of for presbycusis the pathogenesis.
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