|
Background: Gliomas are the most common malignant brain tumor, rapid progression, early No obvious clinical manifestations, most patients visit in late inherently invasive growth characteristics, and normal brain tissue boundaries, the majority are not limited to the lobes of the brain, surgery often can not be full cut, even though the auxiliary to radiotherapy and chemotherapy, its efficacy is still poor, still higher recurrence rate and mortality, serious harm to human health. Early detection and identification of glioma glioma therapeutic significance. With the development of imaging, clinical diagnosis of glioma has made great strides, but the diagnosis of glioma biology is still a lack of effective means, the main reason for this situation is caused by glioma lack of sensitive and specific biomarkers. In recent years, the rapid development of proteomics to find new glioma markers provide a new technology platform. Early diagnosis and differential depends on glioma specimens, plasma, cerebrospinal fluid specific tumor biomarker discovery. Glioma little serum protein expression profiling studies at home and abroad, may be due to the blood - brain barrier to cause glioma-specific proteins in the serum expression rarely. Metabolites of glioma cells directly into the cerebrospinal fluid, cerebrospinal fluid protein-specific changes dynamically reflect the metabolic state of glioma, so the protein components in cerebrospinal fluid analysis may find biomarkers of glioma characteristic thereof. Glioma biology markup study, however, the most widely used and tumor specimens or cell lines analyzed, to find tumor diagnosis of benign and malignant the distinction tumor markers. Malignant glioma cell lines proteomics research as much as possible to find the specific proteins involved in the Taiwanese glioma evolution will help to further clarify glioma pathogenesis find therapeutic targets. clinical drug screening and research and development of new drugs. Objective: To establish people with malignant glioma cell lines Proteome expression profiles provide the necessary reference to the research as the basis. As much as possible to find the key proteins involved in the Taiwanese glioma evolution, and provide a theoretical basis for elucidating the pathogenesis of gliomas, looking for therapeutic targets, clinical drug screening and new drug development and data reference. : Extract three cases of people with malignant glioma cell line CHG-5, TJ899 and TJ905 total protein ratio mix underwent two-dimensional electrophoresis, normal glial cells in parallel control, screening and identification of differential protein spots, and which part of the protein cells immunochemical method validation. Results: A total of 13 different protein spots, screened 5 protein expression decreased 8 expression level rise, of which 10 were successfully identified, mainly belonging to the cytoskeletal proteins, metabolic-related protein, tumor cell migration, as well as stress and The inflammatory response associated protein. Immunocytochemistry test results with the results of proteomic techniques similar. Conclusion: people with malignant glioma cell lines and normal glial cells has multiple distinct differences in protein expression, they may be involved in brain glial cell neoplasia.
|