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Methylation Alterations of miR-34 Gene and its Significance of Hazakh’s Esophageal Squamous Cell Carcinoma in XinJiang Province

Author: LiuDong
Tutor: LiFeng
School: Shihezi University
Course: Pathology and Pathophysiology
Keywords: Esophageal squamous cell carcinoma miR-34b / c Methylation MALDI-TOF MS
CLC: R735.1
Type: Master's thesis
Year: 2010
Downloads: 40
Quote: 1
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Abstract


Objective: detection of the Xinjiang Kazakh esophageal cancer miR-34 gene methylation status, and to explore the relationship between miR-34 gene methylation and Xinjiang Kazak esophageal cancer and its significance. Methods: Using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) technology to detect 145 cases of esophageal squamous cell carcinoma in Xinjiang Kazakh, 60 cancer adjacent non-cancer tissue and 39 cases of normal esophageal mucosa miR-34 gene methylation status ; analysis of miR-34b / c gene methylation distribution in the three groups of samples using Cluster3.0, TreeView, and GraphPad Prism 5.0 software; SPSS 17.0 software to complete the statistical analysis. The results (1) (?) MiR-34b / c gene in esophageal squamous cell carcinoma in Xinjiang Kazakh methylation level higher than in adjacent non-cancerous tissue and normal esophageal mucosa. (2) of miR-34b / c gene 8 CpG units in the of 5 CpG flats (CpG 1 .2.3 the CpG 9 .10, CpG 1 < / sub> 1.12.13, CpG 1 4, CpG 1 5.16) differences in methylation in the three groups of samples (esophageal gt; paraneoplastic gt; Normal) statistically significant (p lt; 0.05). (3) comparison of esophageal different degree of differentiation, (?) NiR-34b / c gene CpG 1 .2.3 in poorly differentiated group in the high rate of methylation in the well differentiated group, and the difference statistically significant (p lt; 0.05); comparison of the different clinical stages of esophageal cancer, miR-34b / the c gene CpG 9 .10, CpG 1 1.12. 13 high methylation in Phase II Ⅱ in Ⅲ stage IV period, and the difference was statistically significant (p lt; 0.05). MiR-34b / c gene methylation with gender, age, tumor location, macroscopic type, lymph node metastasis, the difference was not statistically significant (p gt; 0.05), clinical stage, and HPV16 infection comparison. Conclusion: (1) Xinjiang Kazak esophageal squamous cell miR-34b / c gene methylation is higher than cancer paraneoplastic and normal controls, suggesting that miR-34b / c gene methylation may be associated with the incidence of esophageal cancer in Xinjiang Kazakh. (2) miR-34b / c gene CpG 1 .2.3 methylation may be associated with the degree of differentiation of esophageal squamous cell carcinoma in Xinjiang Kazakh, the high rate of methylation in poorly differentiated tumors. MiR-34b / c CpG 9 .10, CpG 1 1.12.13 methylation may Xinjiang Kazakh esophageal squamous cell carcinoma clinical stage tumors as early as medium-term rate of methylation, suggesting that it may be related to tumor early progress.

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Esophageal tumors
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