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The Mechanism That Fangchinoline Inhibits Proliferation of PC-3 Cells

Author: HuangJianGuo
Tutor: HeGuangYuan
School: Huazhong University of Science and Technology
Course: Biopharmaceutical engineering
Keywords: Fangchinoline Prostate cancer PC-3 cells Nude mice model Apoptosis
CLC: R737.25
Type: Master's thesis
Year: 2010
Downloads: 30
Quote: 0
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Abstract


Tetrandrine (fenfangji) the Radix Stephaniae Tetrandrae the alias Han Housing has, Gilg, root room has plants stone toad Stephania Tetrandra S. Moore. Contains a variety of isoquinoline alkaloids, mainly medicinal ingredients the of tetrandrine (tetrandrine), and fangchinoline (fangchinoline). Its main effect is favorable water swelling, chills and pain. For edema, beriberi, negative urine, the Fengshibitong eczema Chuangdu, hypertension. Present study found The tetrandrine significant inhibition of tumor cell proliferation, and therefore has the value of drug development. However, its structure is very similar to the fangchinoline anti-tumor research is relatively little, this article will be of human prostate cancer PC-3 cells, research materials, application of RT-PCR, Western blotting was nude mouse model experiments Menispermaceae the Norlin alkali impact on the proliferation of PC-3 cells, the major findings include the following aspects: 1) fangchinoline in a dose-and time-dependently inhibited the proliferation of prostate cancer PC-3 cells. MTT assay, respectively 10,20,30 μm / ml fangchinoline processing of PC-3 cells 1-4 days, 30μm/ml fangchinoline cells 4 days after treatment the best inhibitory effect, reaching 86% inhibition rate; study found 30μm/ml fangchinoline deal with PC-3 cells after 1 day, PC-3 cells were blocked in G1 / S phase of the cell cycle. Western blotting results further confirmed fangchinoline can regulate the cell cycle in PC-3 cells associated protein of Cyclin D1 and p27 and PCNA expression. Cyclin D1 and PCNA 30μm/ml fangchinoline deal with PC-3 cells after 1 day, involved in cell cycle G1 / S phase decreased, while the amount of p27 protein expression inhibited cell proliferation significantly rise. 2) Western blotting analysis of PC-3 cells in the cells involved in apoptosis related proteins after treatment fangchinoline, change. PC-3 cells after 1 day in 30μm/ml fangchinoline processing, intracellular pro-apoptotic proteins Bax and activated Caspase-3 protein expression levels have increased significantly, while the anti-apoptotic Bcl-2 protein expression significantly decreased. 3) We build the nude mouse model to further verify the fangchinoline inhibit tumor growth effect, the results show a continuous process 16 days tumor growth in nude mice was significantly inhibited, and the inhibition rate was 57.8% with fangchinoline. Western blotting detected cell cycle regulatory proteins, and apoptotic protein expression levels change significantly. Cyclin D1 and PCNA mRNA and protein levels are decreased, and p27 protein levels increased significantly. Promote apoptotic proteins Bax and activation of Caspase-3 protein expression also increased significantly, while inhibition of apoptosis protein Bcl-2 decreased. The above results show that fangchinoline might become the treatment of prostate cancer of a potential drug.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Male genitalia tumors > Prostate cancer
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