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Chinese medicine is a treasure of China's five thousand years of civilization. According to statistics, China Chinese exports accounted for only 3% of the global medicine trade, the key reason is the lack of a systematic study of modern science and analytical tools, making the original drug quality inspection, the active ingredient extraction, preparation of quality control, efficacy pathological Toxicology are behind the developed countries. How to combine modern means of instrumental analysis, a more systematic and in-depth fundamental research of traditional Chinese medicine material, will be an important task for future drug analysis. Salvia for Labiatae (salivia miltorrhrza Bge.), Dried roots and rhizomes, promoting blood circulation, soothe the nerves rather heart, pain and other effects, in recent years, is widely used for the treatment of coronary heart disease, angina and pyrogenic infection. Its main active ingredient is a water-soluble phenolic acids and fat-soluble the diterpene wake substances. Pharmacological experiments show that the water-soluble phenolic acids, protocatechuic aldehyde, protocatechuic acid as the main active ingredient of the anti-angina, with a significant increase in coronary blood flow and against platelet aggregation induced by ADP (adenosine diphosphate); fat-soluble active ingredients tanshinone Ⅱ A, which not only has a natural antioxidant, anti-atherosclerosis atherosclerosis, reducing myocardial oxygen consumption and other cardiovascular pharmacological effects and anti-bacterial anti-inflammatory effect, but more importantly, has significant anti-tumor effect. Protocatechuic aldehyde, catechin, tanshinone Ⅱ A as the research object, do the following aspects: the first part of the paper by capillary electrophoresis - amperometric detection (CE-ED), the working electrode is 0.3mm carbon round disk electrode of the three-electrode system, when the capillary length is 65cm, and the separation voltage of 24kV in the best electrophoretic medium (pH = 6.4,15 mmol / L phosphate buffer solution), separation of water-soluble components protocatechualdehyde Salvia and the original catechin. The reproducibility of the method, high sensitivity, wide linear range for the detection of Salvia and its two proprietary Chinese medicines protocatechuic aldehyde and catechin, to obtain satisfactory results. For more in-depth study of the first part of protocatechuic aldehyde carbon electrode electrochemical behavior, the second part of this thesis using linear sweep voltammetry (LSV), cyclic voltammetry (CV), differential pulse voltammetry ( DPV) on the place tea aldehyde on the glassy carbon electrode electrochemical redox behavior of a detailed study of the electrode reaction mechanism is discussed preliminarily. Protocatechuic aldehyde was determined sensitive electrochemical analysis method, established on the basis of this analysis of the the original analog serum catechu aldehyde, with good results. Salvia proprietary Chinese medicines preparation process is different: take the ethanol extract, made from the extract of proprietary Chinese medicine retains almost all of the fat-soluble ingredients; taken boiling water extract of proprietary Chinese medicines, lose almost all of its fat-soluble ingredients. Therefore, looking for a simple, rapid determination of Salvia its proprietary water-soluble and fat-soluble ingredients, fully guarantee the quality and clinical effect of the drug can not be underestimated. The third summary points for the first time using micellar electrokinetic capillary chromatography Yang ECC into pressure injection u. SPSI, SS separation voltage of 15kV, uncoated silica capillary (75op-c50cm, effective length 40cm), in 20mof / L NaH. PO. A Na. The B4Q the buffer (pHS.63) a 60nunol / L 10 H a 15 mmol / L sodium cholate (SC), sodium dodecyl sulfate (SDS) electrophoretic medium separation and determination of both Salvia and its proprietary Chinese medicines, analog serum water-soluble components protocatechualdehyde original catechin and fat-soluble ingredients the tanshinone * A Salvia and its compound preparation of water-soluble and fat-soluble components of quality control to establish a new experimental method.
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