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Homogeneous electrogenerated chemiluminescence immunoassay

Author: QiHongLan
Tutor: ZhangChengXiao
School: Shaanxi Normal University
Course: Analysis of bioelectrical
Keywords: Electrochemiluminescence Drugs Luminol Lucigenin Homogeneous electrochemiluminescence immunoassay
CLC: O657.3
Type: Master's thesis
Year: 2002
Downloads: 249
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Abstract

This thesis is composed by the two parts of the review, research reports. The first part includes the history of the immunoassay, electrochemiluminescence immunoassay principle and characteristics, the electrochemiluminescence immunoassay labeling technique, the main types of electrochemiluminescence immunoassay, in recent years electrochemiluminescence immunoassay in its power The chemiluminescence immunoassay six parts of the new method. The second part of the luminol and lucigenin electrochemiluminescence immune response before and after the use of molecular diffusion coefficient based on the electron transfer coupled electrochemiluminescence method for the determination of procaine hydrochloride, isatin, vitamin B2; changes and competitive immunoassay, the establishment of a multi-label homogeneous electrochemiluminescence immunoassay to digoxin, luminol and BSA as a model, for the determination of anti-digoxin antibody and digoxin content electrochemiluminescence immunoassay. The electrochemiluminescence is applied to the electrodes a certain voltage to an electrochemical reaction, the reaction product, or between the reaction product with some component of the system is a chemical reaction, with a photomultiplier tube, such as conventional optical means of measuring the luminescence spectrum and intensity the substance may be a method for trace analysis. Simple the electrochemiluminescence its analysis method, high sensitivity, good selectivity, controllability etc. are widespread concern. Since the 1980s, the electrochemiluminescence is applied to the practice, began the study of the immune electrochemiluminescence and bioluminescence. In the nineties, the improvement of the finished instrument and electrode materials, broadened electrochemiluminescence analysis applications. Electrochemiluminescence with a variety of techniques such as high performance liquid chromatography, capillary electrophoresis, magnetic particles has been widely binding. The electrochemiluminescence researchers committed to the the electrochemiluminescence new system, electrochemiluminescence biochip development, the electrochemiluminescence immunoassay continue to improve, electrochemiluminescence with other technologies to further integrate research. The overview section of the paper on the history of the immunoassay, electrochemiluminescence immunoassay analysis principles and characteristics, the electrochemiluminescence immunoassay labeling technique, the main types of electrochemiluminescence immunoassay, electrochemiluminescence immunoassay in the analysis and its power The chemiluminescence immunoassay comments. Introduces recent years electrochemiluminescence immunoassay, electrochemiluminescence - luminol system, ruthenium bipyridine, acridinium system in the immunoassay are summarized. The research report consists of four parts. The first three research report is built based on the electron transfer coupling electrochemiluminescence. Use of the existing of luminol and Lucigenin electrochemical light emitting system, determination of procaine hydrochloride, indole quinone, vitamin B2. New method for determination of procaine hydrochloride, procaine hydrochloride ECL of luminol enhanced role, the establishment of luminol electrochemiluminescence. Under optimum conditions, procaine hydrochloride in which 4.0 × 10-7g/mL ~ 6.0 × 10-6g/mL had a good linear relationship, the linear regression equation was ΔI = 303.7C-23.7 (C units of 10-6g / mL), a correlation coefficient of 0.9984. According to IUPAC rules, the detection limit was 2.0 × 10-7g/mL (S / N = 3). On a 1.0 × 10-6g/ml procaine 11 measurements, the relative standard deviation of 4.4O. Can be used for the determination of the injection sample. Take advantage of the the lucigenin system of electrochemiluminescence, the main building of the electron transfer coupling electrochemiluminescence determination the call microphone awake and vitamin BZ. The electrochemiluminescence roar microphone stuffed the constant potential -0 Stone SV under Lucigenin strongly enhanced the measured La foot hold in the mouth acid analysis based on established methods. At 4. SX10 fly / L * .9 X10-'g / mL of the range of concentration blanket microphone wake up with the relative electrochemiluminescence value a good linear relationship, Whoa! The microphone wake detection limit of 3.3 XIO-' g / IL, called Mi wake of IXIO fly / mL of 11 determinations, the relative standard deviation of 3.8O vitamin BZ potentiostat {.65 V the under Lucigenin power {dagger Xuefa first strong enhancement Based on this, vitamin BZ quantitative determination of optimum conditions found in the 2.IX10 / mL-3 knife XIO-fly / mL vitamin BZ good linear relationship between Fat strength, linear equations NL-187 +112 C (in units of 10 {g / mL, r 0.9968). According to the provisions of the IUMC, Sp3, vitamin B. The detection limit of 8.2 xlo / mL, and the relative standard deviation of 4% by knife (n SECRETARY, C = 8 knife xlo sg / ml) can be used for injections and tablets of vitamin B content Determination of the advantages of the above three-part experiment is: 1 based on electronic transfer coupling electrochemiluminescence points folding establish a new method for determination of drug; 2 electrochemiluminescence studied in neutral aqueous solutions, media conditions ease. fourth part is using electrochemiluminescence of luminol system, to explore the feasibility of multi-label homogeneous electrochemiluminescence immunoassay digoxin, luminol and BSA model, a determination of anti-digoxin antibody and digoxin content homogeneous the electrochemiluminescence immunoassay new method. luminol and digoxin subscript i have BSA, a pick can mark more than one A luminol and digoxin, improved luminous efficiency, but also can reduce the detection limit the use of the immune response before and after changes in molecular diffusion coefficient against digoxin antibody and digoxin were measured in selected experimental conditions, when the concentration of the marker compound for 5.0 xlo a from / mL (digoxin concentration), Antibody in 300-50000 dilution Q changes when the electrochemiluminescence signal showed a decreasing trend. fixed subscript j has digoxin concentration of the antigen was 5.0 XIO / mL solution of anti-digoxin antibody of the diluted 5000-fold, the digoxin concentration and electric-inch dagger to learn the first intensity linear relationship between 0.sng/mL-30ng/mL, the detection limit was 2.8 x10'' g / mL. On L. 0 xlo 'eggs L digoxin mouth determinations, relative standard deviation of 5.l0. Determination of digoxin content in the serum samples, test results show that the proposed multi-label homogeneous electrochemiluminescence immunoassay method is feasible design of this part of the experiment has the following advantages: l subscript i had a homogeneous electrochemiluminescence immunoassay; 2 is introduced in a small intermolecular macromolecules, certain small molecules can be labeled in a macromolecule 3 small molecules with large egg improve the subscript j has, but also improve the sensitivity of the method;

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