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Bio-metallurgical technology has the full use of resources, low cost, less investment and almost no environmental pollution, both at home and abroad, has been extensively studied and applied to industrial practice. Arsenic copper sulfide ore, iron oxide thiooxidans can oxidize Fe 2 sup> for the Fe 3 sup> biochemical characteristics, gradual increase in solution Fe 2 sup> the initial concentration of the bacteria domesticated, domesticated after liquid containing high concentrations of Fe, and then as the leaching agent to oxidative leaching of copper sulphide ore As of Cu and other elements. Independent of the oxidation process of the experimental design, the ore leaching of bacterial oxidation process to isolate the purpose of the oxidation conditions to achieve the best, to avoid bacteria may cause interference, and arsenic toxicity due to the complex composition of minerals adverse impact. After training for acquisition to the native bacterium as bacteria, in a certain pH value, temperature, and concentration of bacteria and other conditions under its acclimation. Acclimation method is divided into three components: the bacteria / sterile oxidation contrast the experimental solution Fe 2 sup> initial concentration gradual rise domestication experiments and constant temperature water bath oxidation experiments. Through the three aspects of the experiment, get the bacteria domesticated conclusion: (1) The activity of good bacteria and adequate supply of oxygen is bacterial oxidation of Fe 2 sup> two important factors; (2) initial pH value of the reaction solution was about 1.5, the temperature is 25-30 ℃, the reaction was carried out for 7-8 days, the solution Fe 2 sup> of the initial concentration does not exceed 90g / L are preferred domesticated conditions; (3) Fe 2 sup> The initial concentration is 30g / L, the oxidation rate of PE in the solution reaches the maximum, 72.33%; leaching test is divided into four steps: using pure 9K domestication broth as the leaching agent leaching; initial [Fe 2 sup>] 20g / l, 30g / l, 40g / l, 60g / l domestication broth as the leaching agent bioleaching ; using the initial [Fe 2 sup>] domesticated 110g / l broth as bioleaching leaching agent; sterile, containing high concentrations Fe 3 sup> of bioleaching leaching agent. The resultant experimental results on the leaching of copper sulfide concentrate containing Fe broth: (1) bacteria / sterile contrast leaching experiments, found that the presence of bacteria fundamentally improve the leaching effect, increase the rate of leaching; ( 2) ore particle size of -200 mesh to -300 mesh ore slurry solid ratio of 20:1 to 30:1, leaching time of about 10 days, the medium of initial pH value of about 1.5 for the optimum leaching conditions; (3) Initial [Fe 3 sup>] was 5.0 to 30.0g / l the leaching rate is high, 6.5g / 1, AS leaching rate of 52.16%; (4) through a variety of copper sulfide the ore leaching mechanism analysis, composite mechanism for the direct and indirect effects of the coexistence to push Jhihben experimental leaching mechanism.
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