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The Effect of Citrate on Osteopontin Expression of Urinary Oxalate Calcium Stone Formation in Rats

Author: CaiChunDong
Tutor: JiangNing
School: Ningxia Medical University
Course: Department of Urology
Keywords: Potassium citrate Osteopontin Kidney stones
CLC: R692.4
Type: Master's thesis
Year: 2010
Downloads: 50
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Abstract


Purpose of osteopontin (osteopontin, OPN) urinary calculi formation of an inhibitory protein, this inhibits stone formation mechanism of action is still controversial. In this study, potassium citrate for kidney stones rat kidney OPN and OPN-mRNA expression, to further explore the relationship between potassium citrate and OPN and OPN-mRNA between, not only to resolve the pathogenesis of kidney stones experimental basis, but also has some way to seek the prevention and treatment of kidney stones in clinical significance. 32 8-week-old male Wistar rats were randomly divided into 4 groups of normal control group, the potassium citrate group, the stone group (ethylene glycol and ammonium chloride), stones potassium citrate group, n = 8, B glycol and ammonium chloride induced renal calcium oxalate stone model were tested. After four weeks of feeding 24h urine EDTA method and colorimetric determination of urinary calcium, urinary oxalate content. The rats were sacrificed renal tissue by HE staining, immunohistochemistry, RT-PCR method to detect the stone formation in rats, OPN and OPN-mRNA expression. Results ① light microscope: stones potassium citrate group and stones rats were stone formation, the stones of potassium citrate rats significantly reduced the formation of kidney stones than the stones group (P lt; 0.05), the control group and the potassium citrate group, no stone formation. ② 24h urinary calcium, oxalic acid content: the group of stones and stones potassium citrate group urinary calcium, oxalic acid content increased significantly, with the control group and the potassium citrate group compared with the significant difference (P lt; 0.05) Stones potassium citrate group than in the group of stones was significantly reduced (P lt; 0.05). (3) the immunohistochemical detection of the expression of OPN results: OPN expression in all four groups, the group of stones with stones potassium citrate group than in the control group and the potassium citrate group significantly increased (P lt; 0.05); stones citrate the rafters potassium rats OPN expression was significantly lower than the stones group (P lt; 0.05); potassium citrate group OPN expression no significant change compared with the control group (P gt; 0.05) (4) RT-PCR detection of OPN-mRNA expression: RT-PCR OPN-mRNA was expressed in all four groups, OPN-mRNA in control group, simple citrate the potassium renal tissue expression weak; stones citrate potassium rats OPN-mRNA expression was significantly lower than the stone group (P lt; 0.05); potassium citrate group OPN-mRNA expression compared with the control group compared to no significant change (P gt; 0.05). Conclusion potassium citrate may reduce the formation of calcium oxalate crystals as well as damage to the renal tubular epithelial cells, reducing the deposition of calcium oxalate crystals in the kidney reduce OPN nucleation sites for adhesion to the surface of epithelial cells damaged, and reduce of kidney stones rat OPN and OPN-mRNA expression, thus effectively inhibit calcium oxalate stone formation in the rat kidney.

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CLC: > Medicine, health > Surgery > Urology ( urinary and reproductive system diseases) > Kidney disease > Kidney stones
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