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Purpose of the experiment, observing nerve regeneration indoor cholecystokinin octapeptide (CCK-8) on the effect of sciatic nerve defects, explore CCK-8 construct artificial neural repair of peripheral nerve defect mechanism, provide the basis for clinical application. Materials and Methods Healthy Wistar rats were randomly divided into three groups: n = 16, experimental group A, B groups: A group of intraductal injection of CCK-8 group, B group was intraperitoneally injected CCK-8 group, control group, group C (intraductal injection of saline), sciatic nerve defects using an experimental model home side PLGA nerve regeneration chamber constructed catheter. A group of rats were injected in the catheter disposable CCK-8 (56nmol/kg), B group, intraperitoneal injection of CCK-8 (8nmol/kg), once daily administration of a total of 7 days, the catheter is filled with one saline solution. One control group C filled with saline in the catheter. Fed rats for 12 weeks. Using a combination of seven indicators of the degree of recovery sciatic assessment: (a) movement behavior: the first eight weeks after surgery, respectively, and 12 weeks, using the naked eye. (2) regenerated nerve morphology: the first eight weeks after surgery, respectively, and 12 weeks, using the naked eye. (3) the recovery rate of sciatic function index (SFI%): using gait analysis method. (4) neurophysiological (NEP): Determination of motor nerve conduction using several recovery rate (NCV%) and gastrocnemius muscle recovery rate of compound action potentials (CMAP%) method. (5) the structure of the regenerated nerve histology comparison: HE staining, solid dyed green myelin regeneration of the sciatic nerve specimens were observed under light microscope. (6) myelinated nerve fiber count recovery rate (%). (7) gastrocnemius wet weight recovery rate (%). Results 48 rats all survived. (1) Movement behavior: after the first eight weeks, three groups were seen in the calf muscle atrophy A, B two large claws that some or closed Shushu and flexion, A group obviously, C group had no more activity performance; after the first 12 weeks, A group of experimental side recovered to normal calf, toe or closed and flexion was normal; B experiment compared with the contralateral side of the calf muscle is different, yet full, or closed toe flexion activities still; C group experimental side marked atrophy calf, toe or closed flexion activity was limited. (2) regenerated nerve morphology: the naked form: after the first eight weeks, A group and B group canal nerve regeneration through nerve punch rough appearance, B group obviously, C group no nerve regeneration through the tube. After the first 12 weeks, A group of nerve fiber regeneration entirely remote from the proximal ingrowth, normal thickness, shape symmetry; B group regenerated nerve fibers from the proximal ingrowth basic remote, small diameter, convex and concave surface roughness. Group C nerve fiber regeneration basic proximal ingrowth from the distal diameter was significantly smaller than the A group, obviously convex and concave surface roughness. Group A than in group B, B group than C group. (3) SFI%: after the first eight weeks, A group: -51.04 ± 6.77%, B group: -61.12 ± 8.77%, C group: -72.87 ± 5.85%; after the first 12 weeks, A group: -34.15 ± 5.57%, B group: -42.29 ± 6.72%, C group: -52.99 ± 7.80%, three statistically significant difference between the groups. (4) NEP determination: after the first eight weeks, A group NCV%: 42.62 ± 0.43%, CMAP%: 64.16 ± 0.98%, B group NCV%: 35.73 ± 0.28%, CMAP%: 55.28 ± 0.89%; C group NCV%: 23.53 ± 0.76%, CMAP%: 34.97 ± 0.71%, the difference between the three groups was statistically significant; after the first 12 weeks, A group NCV%: 57.23 ± 0.43%, CMAP%: 92.60 ± 2.63%; B group NCV%: 36.26 ± 0.29%, CMAP%: 83.40 ± 3.00%; C group NCV%: 23.50 ± 0.61%, CMAP%: 64.01 ± 1.28%; three statistically significant difference between the groups. (5) Histological structure: regenerated nerve fiber density level, the degree of alignment rules compared to the first eight weeks after surgery, A, B, C group density and arrangement of regularity in descending order; after the first 12 weeks, regenerated nerve fibers arranged rules degrees, with relatively thick myelin, A, B, C group arranged regularly degrees, followed by decreasing the thickness of the myelin sheath, A group of normal. (6) regenerated myelinated fiber count recovery rate measurement: after the first eight weeks of group A was 63.24 ± 6.65%, B group 52.08 ± 3.37%, C group was 37.69 ± 6.37%, three statistically significant difference between the groups. After the first 12 weeks: A group was 81.59 ± 3.24%, B group 72.48 ± 5.37%, C group was 62.37 ± 7.91%. (7) gastrocnemius wet weight recovery rate measurement: after the first 8 weeks: A group was 65.45 ± 1.20%, B group 60.50 ± 1.44%, C group was 53.05 ± 0.74%, three statistically significant difference between the groups; surgery After 12 weeks, A group of 78.14 ± 0.38%, B group 66.32 ± 1.70%, C group was 54.62 ± 1.04%, three statistically significant difference between the groups concluded a topical application of nerve defect side than intraperitoneal CCK-8 injection, can be more effective in promoting peripheral nerve regeneration, is more conducive to the recovery of motor function. 2 clinical study will be used as artificial neural CCK-8 materials (ie composite tissue nerve conduit) repair of peripheral nerve defects provide an experimental basis.
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