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Experimental purposes gastrointestinal ischemia-reperfusion injury of gastrointestinal surgery, systemic inflammatory response syndrome and physical stress cause gastrointestinal ulcers, bleeding, or dysfunction of a basic pathophysiological changes. The subject using immunohistochemical detection method, observation of gastric ischemia reperfusion period of time after 30min different gastric mucosal expression of p38MAPK (mitogen-activated protein kinase, MAPK) and caspase -3 (caspase-3 last) trend; combined with the corresponding period of time gastric mucosal injury index and its pathological changes, discover gastric ischemia-reperfusion injury (gastric ischemia-reperfusion injury, GI-RI) law of p38MAPK and caspase-3 expression; medical statistics analysis p38MAPK caspase-3 expression and gastric mucosal injury degree, and to explore the relationship both expressed in the GI-RI. Materials and methods healthy adult SD rats, male and female, weight 220 ± 10g were randomly divided into sham operation group and ischemia-reperfusion group. 10% the chloral hydrate 0.3ml/100g intraperitoneal injection, after the success of anesthesia for abdominal incision, isolated and blocked the main artery of the stomach (left gastric artery, right gastric artery, the left gastroepiploic artery, right gastroepiploic artery and stomach , short gastric artery) the artery clip perfusion 0.5h, 1h, 3h, 6h and 24h, off cervical rats were sacrificed, open stomachs removed after 30 min. First two forceps clipping the end of the esophagus and duodenum, stomach, and then intragastric injection of 10% neutral formalin solution 1ml, stomachs: cut along the greater curvature of stomach wale, ice brine into the 10% neutral formalin solution measuring gastric bleeding, labeled, placed in 10% neutral formalin fixed about 12h; drawn mainly take the glandular stomach tissue of approximately 0.5 x 1cm2, embedded in paraffin, making 5 serial sections (each sample to make three tissue sections were used for p38MAPK, caspase-3 detection and HE staining). By immunohistochemical method detected 30 cases of gastric p38MAPK and caspase-3 expression as a control, sham-operated group, 400 × microscope, each slice randomly selected five fields in the gastric mucosa area. Application IPP6.0 image analysis software detects integral optical density (IOD) and area (Area) value calculable mean optical density (mean density), the measured data are presented as mean ± standard deviation. SPSS 13.0 software on the analysis of relevant data, take p = 0.05 significance level. Sham-operated group mucosal expression of p38MAPK and caspase-3 level is lower, a rapid increase in gastric mucosal expression of p38MAPK and caspase-3 level in the GI-R; reperfusion 0.5h caspase-3 expression was the peak, and then begin to decline, to re- 3h after reperfusion minimize, and then slowly increased to 24h of reperfusion up to the second peak; p38MAPK reperfusion 1h, peak, and then decreased, but remained at a high level to reperfusion: 24h. GI-R gastric mucosal injury index increased significantly to reperfusion 1h reached its peak; With the prolongation of reperfusion, the gastric mucosa repair or adapt to a variety of factors, damage index began to decline, to reperfusion 3h to trough; Since then the damage index began to rise slowly, gastric mucosal injury, there are heavier but less than the former, at 6h after reperfusion to 24 h did not change significantly. Conclusion GI-RI is by a number of factors involved in the injury and repair process, caspase-3 expression is an important factor in promoting GI-RI progress in the progress of the GI-R 1H; GI-RI fastest, the most serious performance, the period of time may be the key to prevention and treatment of GI-RI's; p38MAPK regulation of the inflammatory response, apoptosis, involved in the formation, development and outcome of the GI-RI's; p38MAPK and caspase-3 expression in the GI-RI does not show statistically significant correlation.
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