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The Biological Characteristics of Bursin of Chicken and Duck and the Expression of Bursin

Author: ZhangHuanLing
Tutor: HaoQinZong;ZuoXiaoLiang;QianJianFei
School: Agricultural University of Hebei
Course: Preventive Veterinary Medicine
Keywords: Chicken Bursin Ap Bursin IBD vaccine Rabbit plague inactivated vaccine Immune response Weight gain Clone Expression
CLC: S852.2
Type: Master's thesis
Year: 2002
Downloads: 105
Quote: 1
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Abstract


Ultrafiltration using a normal chicken, duck bursal tissue extract substances of a molecular weight of less than 1000Da, whose main component is detected by thin layer chromatography and high pressure liquid chromatography, respectively, prove that the method of two ultrafiltrate's capsular active peptide (bursin and tri-peptide, bursin, BS) the presence of, respectively, calculated of bursin Chicken, Duck bursal Extract of tripeptide content. Tlc method BS content of the extract obtained bursal 1.436mg/mL, the BS content Ap bursal extract 4.035mg/mL; high pressure liquid chromatography method obtained Chicken law's of capsular extract paste content 1.199mg/mL, BS Ap bursal extract content of 3.316mg/mL. Bursin obtained by the ultrafiltration intramuscular injection of different doses of 21-day-old SPF chickens, subcutaneous injection IBD embryo toxicity or cytotoxic inactivated vaccine, the results showed that: chickens, ducks Bursin are able to shortening oil IBD vaccine induced antibodies time, to improve the level of antibody, so that the chickens can be obtained within a relatively short period of time a strong immune. The 0.4mLCBS embryo poison group 0.8mLCBS of cytotoxic group or 0.8mLDBS embryo toxicity and cytotoxic group AGP average antibody titer immune control group 2 titer. Bursin with IBD live vaccine combined immunodeficiency chicken, the results show that: different doses of CBS and DBS can be different day-old chicken IBD antibodies have different degrees of role; also can reduce the attenuated chicken bursal tissue damage, accelerate its repair. The tests also found that, BS on the chicken body weight gain thereby improving the feed conversion. Different doses of BS rabbit plague inactivated vaccine immunizing rabbits with high doses of DBS, CBS group can make the rabbit plague HI antibody levels 2-3 titer higher than the immune control group. T-lymphocyte transformation test: BS short promote T-lymphocyte activation. By weight gain proved BS mammals with increased body weight. Although already quite extensive and in-depth research on the BS, but is still a blank in the area of ??molecular biology, in particular the cloning and expression of the BS, and abroad so far not yet been reported. This test is the first attempt for 5 BS gene tryptophan series cloning, expression, and success fully proves that the the BS gene can be amplified in E. coli expression thereby obtaining purified BS. But the whole expression system is still not perfect, still need further research and exploration.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Basic Veterinary Science > Animal physiology, biophysics,biochemistry
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