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Ca~(2+) in Relation to Reaction and Adaptation of Wheat under Osmotic Stress

Author: ZhengGuiZhen
Tutor: LiGuangMin;GuanJunFeng
School: Hebei Normal
Course: Botany
Keywords: Wheat Osmotic Stress Ca2- CAM system Drought resistance Peroxidase
CLC: S512.1
Type: Master's thesis
Year: 2002
Downloads: 120
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Abstract


The trial of winter wheat (Triticum aestivum L.) 4185 to test materials to study the infiltration of normal water supply and 20% PEG-6000 (-0.49MPa) stress under different concentration free Ca 2 , EGTA, LaCl < sub> 2 , Verapamil and CPZ, TFP wheat shoots and root growth and POD, and osmotic stress the CaM level changes; penetration of 25% PEG-6000 (-0.73MPa) Stress LaCl 3 CPZ, TFP, CaCl 2 and EGTA of wheat seedling water content, membrane permeability and growth of the role of the simultaneous determination of wheat seedlings under osmotic stress calcium and CaM content changes, the results are as follows: osmotic stress 10 -10 7 mol / L of Ca 2 wheat young beneficial to the growth of shoots and roots, 10 ? and 10 ? mol / L of Ca 2 inhibition of wheat shoots, root growth, osmotic stress 10 mmol / L EGTA pretreatment of wheat, 1 mmol / L the CaCl of 2 so that growth was partially reversed, 5mmol / L and 10 mmol / L of CaCl 2 inhibit their growth, which indicates that only a suitable concentration of Ca 2 fishes advantageously the growth of wheat, the the Ca 2 concentration is too high, it will inhibit the growth of, and penetration Stress wheat shoots, the the radicle exogenous Ca 2 dependent enhancement reflects the enhanced role of calcium on Wheat Drought. 2 under osmotic stress, EGTA (1, 5, 10 mmol / L), the LaCl 3 (0.5, 1 mmol / L), Verapamil (250, 500 mu mol / L) treatment inhibited wheat shoots and root growth, and a significant concentration effect, the greater the concentration, the greater the degree of inhibition. When the normal supply, 1 mmol / L EGTA and 250μ mol / L Verapamil the growth of shoots of wheat without affecting or slightly role, these results indicate that, under osmotic stress, wheat more sensitive to calcium deficiency, reduction of extracellular Ca < sup> 2 or blocking Ca (2) absorption, inhibit the growth of wheat, indicating the importance of calcium to resist drought on wheat. 3 osmotic stress, CPZ (25,50,100,200 μ mol / L) and TFP (10,25,50,100,200 μ mol / L), wheat shoots, inhibit the growth of wheat roots and shoots, and showed significant concentration effect, the greater the concentration, the higher the degree of inhibition; normal water supply, 25 micron mol / L of CPZ and 10μ mol / L TFP growth of wheat shoots and roots had no effect or promote, show that under osmotic stress, inhibition of the activity of CaM negative growth of wheat, wheat drought. 4 osmotic stress, compared with the control, wheat shoots CAM content reduce stress 6h, and then gradually increased, and the peak of CaM in peak time and bud growth, and the roots of CaM content in most published 24 The h 100 low-Ding control increased to 36 hours after the high level of control c These results suggest that inhibition of shoots and roots of a small branch HM Taiwan into the early osmotic stress may be one of the signal in drought stress response, hills and, maintain normal Ca \\ 1 function is to maintain the plant drought resistance Dog important. 5 osmotic stress Bu the exogenous free *, \the amount of K Ah significant or highly significant negative correlation Gang, the endogenous Ca'-Ca \\ l messenger system pattern may POD, then by adjusting the V shadow Diao small branch bud drought. 6 different bite G-6000 penetration Stress showed that 10 'of the PEG-6000 i.e. inhibition of the calcium content of the the small rootlets sheep leaf, and sting Pat concentration increases, lime degree of inhibition of calcium absorption; x'; Wang G-() O processing, small support immature white calcium content compared with the control spit in the 12 Bu Chuan yao, gradually increased in the 24-ish justice,] h drastically reduced and lemon root m's most # of leaves the smallest change. 7 penetration stress compared with the control, and called chip [J content Ah different trends in small branch roots, leaves CaM the stress IZh when rapid rise, and then the land is low, and the roots of CAM in the less stress 12h irk, and then gradually increase to 72h when reduced to low Ding control the level of (fathom stress Bu main root, mouth ten Ca \\ l white lights heterosexual c 'leaves Ca \\ elevated facilitate root C a \\ 1 is reduced, may be young by an 8 i of the drought signal B \u0026 through the stress - Bu, Lin. the of.L lacI. 100 u of'L CPZ, 100 u of l of TFP 10mmolL CACI., and sin. L'L gi EGTA relative water content of the leaves of wheat seedlings and root absolute water content have different degrees of reduction This result suggests that [the '-Cd messenger system involved in the penetration threat Wei Bu support seedling water level regulation of the street. 9 penetration W forced Bu, 1 * Bu L L CI, 1 ARMED Oil CR 1b O1L HCI and 5 O IL the EGTj \\ handle the increased Mo invasion of the cells of the leaves and roots of a small branch young song B wins, the small branch seedlings increase in the extent of injury, indicating that Ca'-Ca \\ messenger Department of pattern involved in cell membrane function adjustable penetration is 10 coercion Bu, Laci, CPZ, the ITP treatment Main seedlings reduced the main port} piece of dry weight, description, C '-CJ Department of pattern adjustment osmotic stress Bu support port f piece raw K, and thus affect the to dry matter accumulation.

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CLC: > Agricultural Sciences > Crop > Cereal crops > Wheat > Wheat
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