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Identification of Protein Expression in Root Tips of Maize Seedling Acclimated to Low-oxygen Stress Environment

Author: LiuXinYu
Tutor: HuangYuBi；ZhaoShiFu
School: Sichuan Agricultural University
Course: Crop Genetics and Breeding
Keywords: maize (zea mays L.) low-oxygen stress 2-DE peptide mass fingerprinting stress protein
CLC: S513
Type: Master's thesis
Year: 2004
Downloads: 210
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Abstract

Oxygen is essential to grow and develop for green plant, and even if rice originated from thermal-wet swampland environment is without exception ,which can not live during the long period treated with anaoxia .maize is the model plant to be used to research oxygen stress. This paper describes the patterns of protein synthesis during hypoxic acclimation. Proteins synthesized most rapidly under normoxic conditions continued to account for most of the proteins synthesized during hypoxic acclimation. While the production of a very few proteins was selectively enhanced, other protein synthesized was depressed and no "new" proteins were detected. The complex and quantitative changes in protein synthesis during acclimation necessitate identification of large numbers of individual proteins. The results show that mass spectrometry can be effectively used to identify plant proteins arrayed by two-dimensional gel electrophoresis (2-DE).The paper describes the expression of proteins acclimated to low-oxygen stress involved in a wide range of cellular functions , including previously reported anaerobic proteins, and discuss their possible role in adaptation of plant to low-oxygen stress.Most of the root tip proteins identified are soluble metabolic enzymes. These included three anaerobic proteins: ADH1 ENOland GAPC . All three proteins showed comparable or increased synthesis during hypoxic acclimation relative. A fourth protein whose synthesis was significantly induced during hypoxic acclimation was tentatively identified as pyruvate decarboxylase(PDC). In addition, we identified protein involved in oxidative phosphorylation(subunits of Fl-ATPase) protein folding(mitochondrial chaperonin) intracellular trafficking(Golgi-associated protein) heat shock protein and P-D-glucosidase (GLU1).For 20 protein spots, identifies were assigned by matching to homologous sequences from other species, which are O.Sativa M.Sativa N .tabacum Arabidopsis H.vulglare> C. roseus G.max B.napus B.vulgarlis.Multiple isoforms of many proteins were identified. Maybe there were due to allelic variation, because I do not know the genetic back-ground of the material. Rather, they may have resulted from post-translational modifications and / or expression ofgenetically distinct isoforms. For example, phosphorylaled proteins are readily separated on two-dimensional gel, due to acidic pI shift.Low-oxygen stress has been shown to trigger many basic cellular responses in plant. Fermentation of sugars plays a crucial role in plant survival under anoxia. actin gene expression was regulated more 2 times higher in hypoxia than in normal. This result shows that actin may be a new stress protein, but which should be verified in more experiments. Our results are consistent with a model in a model in which these enzymes play a role in the root tip acclimation response. The complexity of the acclimation response described here argues against a simple relationship between the level of expression of any single gene and hypoxic tolerance. Rather, multiple suites of gene products may combine to provide tolerance, and the understanding of this products requires analysis of global patterns of gene expression.

Identification of Protein Expression in Root Tips of Maize Seedling Acclimated to Low-oxygen Stress Environment

Abstract

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