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The Heterogeneity of Mdm2 and p53 Binding Proteins in Two Human Glioma Sublines

Author: YuLiNa
Tutor: ZhongXueYun
School: Jinan University
Course: Pathology
Keywords: Glioma Heterogeneity wins Co-immunoprecipitation Mdm2 p53
CLC: R739.4
Type: Master's thesis
Year: 2004
Downloads: 64
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Abstract


The purpose of: (1) to Mdm2 and p53 as a target to explore the human brain glioma cell line SWO-38 two sublines SWOZ1 and SWOZ2 heterogeneity of protein molecular mechanisms; (2) to explore co-immunoprecipitation technique in tumor heterogeneity The feasibility study of protein molecular mechanisms, to establish a new research method for tumor heterogeneity. Methods: (1) using immunohistochemistry to detect human glioma cells SWO-38 and its two sublines SWOZ1 SWOZ2 Mdm2 and p53 protein expression site and level; (2) further testing by Western blot SWO- Mdm2 and p53 protein expression levels of 38 and its two sub-strains; (3) application of immune precipitation technique to detect two sublines SWOZ1 the SWOZ2 in Mdm2 and the p53-related protein expression profiling differences. Results: (1) Mdm2 and p53 in human glioma cells SWO-38 and two sublines SWOZ1 of, SWOZ2 the nuclei were positive expression, and the expression levels of three fairly; (2) SWO-38 and its two sub-lines are detected Mdm2 and p53 protein's expression, and the expression of a considerable; (3) in Mdm2 immune total precipitation material in, Mr 37000 Chu have a protein bar with SWOZ2 obvious deep dyed in SWOZ1; immunity of p53. electrophoretic patterns in the sediment, the same can be clear to see with both Mr 39000 and 38000 two protein bars the SWOZ1 deep-dyed in SWOZ2; and while Mr 37000 protein bar with SWOZ2 obviously deeply stained SWOZ1. Conclusion: (1) the person the glioma cells the two sublines SWOZ1 and SWOZ2, in Mdm2 pathway Mr approximately 37,000 protein expression was significantly different; of p53 pathway Mr approximately 39000,38000,37000 three protein present in an amount significantly different expression in the two sublines. Such differences in protein may play an important role in glioma heterogeneity generation and two sub-strains of cells may determine the performance of different biological behavior of the key. (2) in Mdm2 and p53 immune coprecipitation was in MR 37000 at the protein are SWOZ2 high expression SWOZ1 preliminary infer both may have a similar molecular weight or structure, the mutual adjustment of the cross point of Mdm2 and p53, this The sequencing steps still need to further characterization. (3) with immune coprecipitation technology to explore the the tumor heterogeneity protein molecular mechanisms is a good way; (4) SWOZ1 and SWOZ2 two cell sublines for further study of glioma development mechanism of the occurrence of heterogeneity prime less Jinan, a Master} 'cargo space papers glioma cells the two sublines MdmZ with p53 protein from heterogeneity analysis Xu

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