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Objective To improve the rabbit VX2 liver cancer, kidney cancer, breast cancer, brain tumor model production methods, to make it easier, save time, and increase the rate of tumor formation. At the same time, explore the tumor model imaging performance. Methods (1) VX2 liver cancer model produced in New Zealand white rabbits were 22, were randomly divided into 2 groups (n = 11), B ultrasound-guided percutaneous 18G needle implantation The VX2 tumor mass organizations method were seeded in rabbit liver left lobe (A group), rabbit liver, the right lobe (B group); after inoculation, one week, two weeks, three weeks, four weeks, respectively, the line color Doppler ultrasound, CT and conventional pathological examination. (2) VX2 renal carcinoma model making New Zealand white rabbits were 22, were randomly divided into 2 groups (n = 11), B ultrasound-guided percutaneous implantation of VX2 tumor block tissue suspension method 18G needle were inoculated in the rabbit left kidney (A group) and the right kidney (B group); 1 week, 2 weeks after vaccination, three weeks, four weeks, respectively, CDFI, CT, and routine pathological examination. (3) VX2 model of breast cancer, making 22 New Zealand white rabbits were randomly divided into 2 groups (n = 11), respectively, using percutaneous 18G needle implanted VX2 tumor block tissue suspension method (A group), cut the local skin VX2 tumor block tissue transplantation method (B group) were seeded in the rabbit chest wall to the left the nipple pad under 2 after inoculation 1 week, 2 weeks, 3 weeks, 4 weeks, respectively CDFI, CT, MR, and conventional pathological examination. (4). VX2 brain tumor model making two New Zealand white rabbits were randomly divided into 2 groups (n = 6), respectively, using minimally invasive positioning skull drilling organization block transplant method (A group: Take first cut into a size of about 1mm3 tumor tissue blocks 1 to 2 implanted pre-drilled hole in the skull) and minimally invasive positioning skull drilling tumor tissue mass suspension injection method (B group: VX2 tumor mass suspension 0.3ml injection inoculated into the pre- lay of the the skull hole). One week, two weeks, three weeks after vaccination, four weeks underwent CT and MR imaging observed neurological signs change, natural death observed histopathological changes. Statistics tumor inoculation success rate; postoperative complications observed and recorded: Tumor general performance and metastasis; animals were observed natural survival; autopsy after death; tumor tissues were fixed in 10% formalin, and pathology. 1 rabbit VX2 carcinoma model: 22 rabbits were found of tumors, the assembly tumor rate 72.7% (17/22), the left hepatic lobe inoculated group (A) the success rate was 90.9% (10/11), the right lobe of the liver vaccination group (B) a success rate of 54.5% (7/11), no significant difference in the two sets of vaccination success rate (P gt; 0.05). Rabbit renal VX2 tumor model: 15 rabbits were found tumor foci the total inoculum success rate was 68.2% (15/22), the success of the left kidney vaccination group (A) was 54.5% (6/11), the right kidney vaccination group (B) the success rate was 81.8% (9/11), no significant difference in the two sets of vaccination success rate (P gt; 0.05). 3 rabbit VX2 breast cancer model: 18 rabbits found that tumor foci total vaccination success rate was 81.8% (18/22), the organization-mass suspension planting method vaccination group (A) the success rate was 90.9% (10/11 ), the tissue blocks transplantation group (B) success rate of 72.7 (8/11), vaccination success rate of the two groups was no significant difference (P gt; 0.05). 4. The rabbit VX2 brain tumor model: five rabbits found tumor foci, total vaccination success rate was 41.7% (5/12) organizational block transplant group (A) a success rate of 83.3% (5/6), tissue blocks suspensions planting group (B) 0 (0/6) success rate, the the AB two groups inoculated with the success rate of a significant difference (P lt; 0.01). Conclusion color Doppler ultrasound guidance percutaneous implantation of tissue blocks rabbit VX2 carcinoma model, rabbit renal VX2 tumor model, the use of tissue mass suspension established by injecting rabbit VX2 model of breast cancer by tissue transplantation establishing rabbit VX2 brain tumor model production process, making it easier to save time: using color Doppler ultrasound, CT and MR scanning motion detection, evaluation of tumors may be more accurate, timely observation of the occurrence and development of tumor foci image changes.
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