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Cellular Basics for the Effects of rSNSR1 and 5-HT2A Receptors on Nociception

Author: DaiFeiHong
Tutor: HongYanGuo
School: Fujian Normal University
Course: Cell Biology
Keywords: rSNSR1 5-HT2A receptor NO CGRP PKCε Co-expression of Dorsal root ganglion
CLC: R363
Type: Master's thesis
Year: 2010
Downloads: 8
Quote: 0
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Abstract


Rat sensory neuron-specific receptor rSNSR1 5-HT2A receptor have been found to play an important role in morphine anti- nociceptive effectiveness of regulation and peripheral inflammatory hyperalgesia . The study pointed out that the dorsal root ganglion cell signaling molecules such as NO, CGRP and PKCε involved in the formation or modulation of pain feelings . This study aimed to explore rSNSR1 cytological basis of these signaling molecules function through the 5-HT2A receptor . Means of experimental application of tissue culture , enzyme-linked immunosorbent assay (ELISA) and immunofluorescence double labeling experiments of rSNSR1 with NO the PKCs relationship and 5-HT2A receptor and PKCε relationship . In vitro culture ; dorsal root ganglia and trigeminal ganglia organization and for four days in a row to give specific agonist BAM8 rSNSR1 - 22 . Observed in the culture medium at the same time to give the non-selective nitric oxide synthase inhibitor L-NAME, will promote CGRP upregulation . Double immunofluorescent labeling results show that the concentration rat DRG , nNOS expression within in rSNSR1 positive cells , these cells for small and medium-sized cells ; PKCε significantly co-expressed with rSNSR1 . 5-HT2A receptor colocalization studies PKCs ,5-HT2A receptor and PKCε coexist in the small and medium-sized DRG neurons . The above experimental results -oriented the previous observed to the chronic activation rSNSR1 after CGRP expression increases morphine analgesic effectiveness decline , and 5-HT2A receptors by activating PKCε pathways lead to inflammatory hyperalgesia findings provide cytological basis .

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