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Toxoplasma gondii (Toxoplasma gondii) obligate intracellular parasitic protozoa , was a global distribution , caused by toxoplasmosis is a very serious harm zoonotic parasitic diseases caused to human health , prenatal and postnatal care , and the development of animal husbandry serious threat. Therefore , toxoplasmosis vaccine has become a top priority of the prevention and treatment of the disease . To clarify the of Toxoplasma metabolic secretory antigen (E / SA) immune effector mechanisms pig , pigs were randomly divided into 6 groups ( n = 5 ) , respectively, for the E / SA group , E / SA the CpG ( not emulsified ) group , E / SA CpG group , E / SA IL -2 group , the E / SA IL -2 of of CpG group and the blank control group . Were immunized twice , every two weeks , two weeks after the second immunization, challenge infection with Toxoplasma gondii GJS strain tachyzoites . Before immunization , 2 weeks after immunization , four weeks after immunization challenge after one week and challenged two weeks after the acquisition piglets peripheral blood , IHA detection reagent to detect the level of anti - Toxoplasma antibodies in the serum ; detected by ELISA serum IL-2, IL-4, IFN-γ and TNF-α content ; dynamic variation of the flow cytometry peripheral blood CD4 and CD8 T lymphocyte subsets . The results showed that anti-Toxoplasma IHA antibody levels after immunization immunohistochemistry most risen 1:1024 ; serum IL - 2 , IL -4 and IFN - γ content of different degrees , wherein the E / SA group IL -2, IL-4, IFN-γ content was significantly increased, its content is elevated to the difference of 194.03,342.50,296.50 pg / mL, and the control group in the immune after significant before the immune 105.05,105.33,67.50 pg / mL , respectively ( P lt; 0.05 ) ; TNF - α content was not obvious . Immunohistochemistry of peripheral blood CD4 and CD8 T lymphocytes after immunization elevated , where E / SA group increased the most obvious , CD4 and CD8 T cell level from 21.51% before the immune increase of 23.9% to the immune 35.72 % and 32.8 %. Toxoplasma gondii GJS virulent attack , immune and control groups pig body temperature differences are significant , immunohistochemistry pig was a transient low-grade fever , normal appetite ; piglets of the control group was missed high temperature , loss of appetite , and associated diarrhea symptoms . The study showed that the E / SA immunized pigs stimulate the body to produce anti - Toxoplasma antibodies , IL - 2 , IL -4 and IFN - γ secretion induce CD4 and CD8 T- lymphocyte proliferation , resulting in a strong humoral and cellular immune response , stimulate the immune response of host resistance to Toxoplasma gondii infection .
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