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The Inducement and Mechanism of Immune Co-stimulatory Molecule B7-H1 by Chemo-preventive Agents in Colon Cancer Cell Line SW480

Author: ChenZhiSong
Tutor: LuXiaoMing
School: Huazhong University of Science and Technology
Course: Surgery
Keywords: Colon cancer SW480 B7-H1
CLC: R392
Type: Master's thesis
Year: 2010
Downloads: 24
Quote: 0
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Abstract


Objective: To study the commonly used chemotherapy drug 5 - fluorouracil and paclitaxel in colon cancer cells induced immune costimulatory molecules B7-H1 expression and mechanism . B7-H1 mRNA was detected by RT-PCR method : 5 - fluorouracil and paclitaxel, SW480 cells 24 hours after treatment , 48 hours later by immunofluorescence and flow cytometry detection of B7-H1 protein . Results: 5 - fluorouracil and paclitaxel in SW480 cells can cause immune costimulatory molecules B7-H1 expression : 5 - fluorouracil (10μg/ml) and paclitaxel (2.5μM) treatment of colon cancer SW480 cells 48 hours available immunofluorescence qualitative B7-H1 protein detected by immunofluorescence signal can also flow cytometry semi-quantitative detection to increase B7-H1 protein . However , semi-quantitative RT-PCR analysis of B7 - H1 mRNA in 5 - fluorouracil (10μg/ml) and paclitaxel (2.5μM) stimulus SW480 cells after 24 hours , and an increase is not like protein . In this experiment, B7-H1 protein increase as a cause of the positive control γ-interferon does not cause the increase in B7-H1 mRNA . Conclusion: chemotherapy drugs in the treatment of cancer at the same time will cause the expression of B7-H1 protein , which may be related to the imprecise chemotherapy treatment , we confirmed that transcription of B7-H1 molecules play an important role in regulation of protein expression .

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CLC: > Medicine, health > Basic Medical > Medical Immunology
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