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Objective: To investigate the role and mechanism of Chinese medicine prevention of POF POF provides theoretical and experimental clinical research data, prevention and treatment of Chinese medicine, this study is the use of modern scientific method, combined with the knowledge of pathology and molecular biology, starting from the immune explore traditional Chinese medicine to protect the eggs soup of autoimmune POF mechanism. Method: 1. Document retrieval method, etiology and pathogenesis, prevention and treatment of POF clinical classification of therapeutic principle as well as the current research progress in medicine, research and analysis. A large number of studies have shown the efficacy of traditional Chinese medicine treatment of premature ovarian failure for sure, and side effects and no drug resistance, is worthy of in-depth exploration of the field. According to the pathological mechanisms of the POF with the mouse zona pellucida the of ZP3 first 330-342 amino acid sequence (NSSSSQFQIHGPR) immunogen to create animal models of autoimmune POF mice, and to intervene to protect the eggs decoction on the use of light microscopy, flow -cell technology, immunohistochemical detection of ovarian morphology changes, CD4 CD25 Treg and FoxP3 expression and change. Results: model group: the gradual emergence of the estrous cycle disorders, relatively early time, prolonged diestrus vaginal smears performance for a large number of white blood cells and a small amount of nucleated epithelial cells; ovarian smaller, less mature follicles, the granulosa cells layer than normal to reduce, disorganized, and part of the follicular dysplasia, ovarian stromal seen more lymphocytes and plasma cells; the peripheral blood AzpAb positive levels; ovarian weight, thymus weight index decreased spleen weight index; CD4 CD25 Treg cells decreased; gene and protein expression of FoxP3 weakened. Protect the eggs soup group: POF mice compared with the model group has improved to some extent, the efficacy of high-dose group than in the low-dose group, the specific performance: estrous cycle disorder time appears relatively late, mainly as estrous cycle extended, reduced estrus keratinocytes, estrous cycle was extended to 8-10d; compared with the model group, ovarian volume slightly larger, more mature follicles. Granulosa cell layer increased, a small part of follicular dysplasia, the ovarian stroma see few lymphocytes and plasma cells. Peripheral blood AzpAb positive, but the levels were significantly lower; ovarian weight, thymus weight index was significantly higher; spleen weight index was significantly reduced; CD4 CD25 Treg cells increased; gene and protein expression of FoxP3 enhanced. Conclusion: The etiology and pathogenesis of POF kidney cell resistance Bushentongluo The basic rule is; close to the experiment to establish the animal model and human POF, POF ideal animal model study; protect the eggs soup of autoimmune ovarian premature aging animal ovarian function, immune function has improved to some extent. The ovarian mass index significantly increased ovarian increased vitality follicle, infiltration of lymphocytes and plasma cells reduced. Sexual cycle regularity, just love interval prolongation; moderating effect on the immune function of the POF. Thymus weight index rose, spleen weight index the, AzpAb potency decline of Treg cells increase in the number of CD4 CD25 gene and protein expression of FoxP3 raised to suppress autoimmune damage.
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