Dissertation > Excellent graduate degree dissertation topics show

Studies on the Isolation, Identification, and Antitumor Bioactivities of Fucoxanthin in Algae

Author: WangShuZuo
Tutor: XueChangHu
School: Ocean University of China
Course: Aquatic Products Processing and Storage Engineering
Keywords: Fucoxanthin Brown algae Diatoms HPLC The anti-tumor activity
CLC: R285
Type: Master's thesis
Year: 2010
Downloads: 261
Quote: 1
Read: Download Dissertation

Abstract

Fucoxanthin is one of the important species of seaweed special carotenoids, anti-obesity, regulate blood sugar, anti-tumor, scavenging free radicals, anti-oxidation, anti-angiogenesis and other physiological activity in human health, poultry, aquatic play an important role. Of algae is rich in resources, mainly fucoxanthin in brown algae and diatoms plant is a kind of a distinguished health food supplements, additives and effective anti-cancer drugs. And diatoms brown algae and its processed products as raw materials, and the establishment of a process of separation and extraction from fucoxanthin; determine the optimum HPLC conditions, the establishment of seaweed, fucoxanthin the HPLC quantitative detection method. The last preliminary study in vitro anti-tumor activity of the isolated and purified from wakame fucoxanthin determine characteristics of fucoxanthin anti-tumor activity. For the future of algae fucoxanthin food (drug) provide theoretical foundation and scientific basis for the development and utilization, and nutritional evaluation. The main contents include the following sections: 1, the establishment of the process of separation and purification of brown algae and diatoms in fucose flavin brown algae and their processed products and three microalgae in fucose flavin content and differences and fucoxanthin isolated structure identification. The separation method is simple, fucoxanthin isolated from the purity of up to 90 percent. 2, for the distribution of pigment in brown algae and diatoms complexity establish a the two detection fucoxanthin HPLC chromatographic conditions. Under the two test conditions, the peak retention time of the fucoxanthin characterized in about 9.5min, peak shape ideal. 3, the establishment of a seaweed fucoxanthin content was measured by HPLC method. This method is a good linear relationship, good repeatability and high accuracy to provide a method for the detection of food materials fucoxanthin content and establish quality standards fucoxanthin. Fucoxanthin vitro antitumor activity conducted a preliminary study, fucoxanthin isolated from wakame antitumor activity screening using the MTT assay. On this basis, the choice of HepG2 cells with morphological observation, AO / EB staining of fucoxanthin in vitro anti-tumor activity and its possible mechanisms; Scratch cell adhesion experiments, Transwell chamber studied fucoxanthin HepG2 cell migration, adhesion, invasion ability of the three aspects; using in vitro tube formation assay the CAM model vitro tumor invasion, metastasis and angiogenesis process, fucoxanthin on tumor angiogenesis. Prove fucoxanthin can inhibit tumor cell proliferation, metastasis, invasion and angiogenesis in the CAM. This article first comprehensive system extracted from the separation, structure identification, content detection and physiological activity of four seaweed fucoxanthin rich fucoxanthin research areas to fill the domestic blank, its future comprehensive utilization of scientific and experimental basis.

Studies on the Isolation, Identification, and Antitumor Bioactivities of Fucoxanthin in Algae

Abstract

Related Dissertations