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Puerarin injection is the main component of the Radix Puerarin injection aqueous solution, is widely used in the clinical treatment of cardiovascular disease. This experiment will establish of puerarin the plasma and saliva samples were determined by HPLC method to study the correlation of puerarin injection of different doses in rats in vivo pharmacokinetics, dynamic observation of puerarin concentration in saliva and blood. The feasibility of therapeutic drug monitoring (TDM) to explore the use of saliva puerarin pharmacokinetic study and clinical application of traditional Chinese medicine injection using saliva to carry TDM to provide a new method, a new foundation. Using saliva on the TDM advantage saliva drug concentration study can directly reflect play a pharmacological effect of the free drug in vivo process. On the basis of experiments, learn the basic theory and method of chemical drugs saliva pharmacokinetic, according to traditional Chinese medicine pharmacokinetic study characteristics, sample handling methods, determination of conditions screening applies to both blood contained traces of puerarin and The saliva sample handling and by HPLC method selectivity, accuracy, reproducibility, high sensitivity, and can meet the requirements of analysis in this study. The sensitivity of the method may be particularly low content of saliva components meet on the saliva of the measured component. Pharmacokinetic study. The rat intravenous high, medium and low dose puerarin injection plasma, saliva samples, collecting Determination of puerarin concentration, pharmacokinetic software 3P97 program processing data, high, medium and low-dose pharmacokinetic parameters are as follows: high-dose: t = 1.188 ± 0.121h, the AUC = 211.052 ± 5.376μg · mL - 1 h, Vc = 2.058 ± 0.260 L · kg - 1 , CL = 0.392 ± 0.030 L. h - 1 ; medium dose: t = 1.066 ± 0.172h AUC = 110.787 ± 0.419μg · mL - 1 h low dose of, Vc = 1.610 ± 0.280 L · kg - 1 , CL = 0.361 ± 0.018L. h - 1 ;: t = 1.043 ± 0.125h AUC = 67.868 ± 10.520μg · mL - 1 . h, Vc = 1.369 ± 0.230 L · kg - 1 , CL = 0.295 ± 0.012 L. h - 1 < / sup>. Submandibular gland and parotid saliva puerarin concentration range in the high, medium and low dose, respectively: 0.15 4 .95,0.31 2 .36,0.15 0 .90 μg · mL - 1 ; 0.20 4 .90,0.43 2 .46,0.17 0 .92 μg · mL - 1 , high, low dose, submandibular gland and parotid correlation coefficient S and P, respectively 0.9539,0.9564,0.8557,0.9479,0.92,0.8346 (P lt ; 0.05), S and P was significantly correlated. Puerarin compare different dose distribution from blood to saliva, each measured ingredients by the blood to saliva transitional quickly, and influenced by the drug itself factors and saliva and salivary glands physiological factors. Free in the determination of the components of saliva concentration and blood drug concentration ratio (C s / C pu) as an indicator of the puerarin blood concentration and saliva concentration correlation analysis, the results showed that puerarin dose within the submandibular gland and parotid saliva concentration of free drug concentration in the blood correlation significant, indicating that the drug concentration in saliva can reflect the free drug concentration levels in the blood, may be considered by the saliva instead of blood puerarin pharmacokinetic study. In this study, the use of saliva drug concentrations instead of blood drug concentration monitoring implementation of TCM injections TDM provides a reference and research base. At the same time broaden the idea of ??Chinese medicine injections TDM, also proved the feasibility of the use of saliva for clinical TDM specific implementation.
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