Dissertation > Excellent graduate degree dissertation topics show

Mechanisms about the Compatibility between Radix Polygalae and Magnolia Which Relievs the Gastrointestinal Motility Inhibition and Gastric Mucosal Injury Caused by Radix Polygalae

Author: XiaoWu
Tutor: WangJian
School: Chengdu University of Traditional Chinese Medicine
Course: Of Pharmacy
Keywords: Polygala Magnolia Compatibility Gastrointestinal motility and mucosal Mechanism
CLC: R285.5
Type: Master's thesis
Year: 2010
Downloads: 42
Quote: 1
Read: Download Dissertation

Abstract


Objective: To investigate Polygala Magnolia compatibility of gastrointestinal neurotransmitters, topical steroid activity, intended to explore the biological mechanisms of Magnolia compatibility mitigation Polygala gastrointestinal motility inhibition and mucosal injury. Methods: ① the colorimetry and nitrate reductase method, respectively, of drug-treated rat gastrointestinal AchE and NO. ② using immunohistochemical methods, examines the impact of drug-treated rat gastric antrum vasoactive intestinal peptide. ③ stereomicroscope, examines the impact of drug-treated mice gastric mucosa. (4) using the TBA method and hydroxylamine method of drug-treated mice stomach MDA, SOD, PGE2 were measured. ⑤ phosphorus method of drug-treated mice stomach Na K-ATPase, Ca2-ATPase. Results: (1) determination of AchE activity results: each dose group of the rat gastric antrum tissue AchE no significant effect (P gt; 0.05); the single herb Polygala significantly reduce the rat duodenal organizations AchE activity (P lt; 0.01), Polygala the compatibility of 1:1, 1:2 and Polygala group with Magnolia compared AchE activity trends, Polygala with the Magnolia compatibility 1:3 group is then able to significantly reduce AchE activity significantly reduce (P lt; 0.05 ). ② NO assay results: Polygala can significantly increase gastric antral tissue NO content (P lt; 0.05) Polygala Polygala group with Magnolia compatibility group, you can significantly reduce the the antral tissue NO content (P lt; 0.05 ), but with the blank control group no significant difference (P gt; .05); each dose group NO content in rat duodenal tissue was not obvious. (3) results of immunohistochemistry: Polygala group the rat antral muscularis's VIP immunoreactive product was significantly enhanced (P lt; 0.01), Magnolia group Polygala with the Magnolia compatibility 1:1 group compared with the control group no significant difference (P gt; 0.05); Polygala the VIP immunoreactive product with Magnolia 1:1 group antral muscularis expression was significantly lower than the single herb Polygala group (P lt; 0.01). ④ gastric mucosa observed experimental results show that: Polygala significantly damage in mice gastric mucosa (P lt; 0.05), Polygala group and the Magnolia compatibility 1:1, 1:2 degree of injury than Polygala group have to reduce Polygala with thick Pak compatibility 1:3 group compared with Polygalaceae group no significant difference (P gt; 0.05). ⑤ SOD, MDA, PGE2 measurement results: each dose group in the mouse stomach tissue SOD, MDA had no significant impact (P gt; 0.05); compared with the control group, Polygala mice stomach tissue PGE2 content tended to decrease , Magnolia Group, Polygala Magnolia compatibility 1:1 group is able to significantly reduce the level of PGE2 (P lt; 0.05), Polygala Polygala group was significantly higher than the level of PGE2 in the gastric tissue in mice, Magnolia 1:3 compatibility group ( P lt; 0.05). ⑥ ATPase measurement results: each dose group Na K-ATPase and Ca2-ATPase in the gastric tissue of mice had no significant affect (P gt; 0.05). Conclusion: ① single herb Polygala inhibit gastrointestinal motility effect may be related to promotion of the antrum of NO secretion, inhibition of AchE activity of the duodenum, and enhanced the antral muscularis VIP immunoreactive products expression related. The of ② Houpu rat gastrointestinal tract NO, of AchE, and antral muscularis VIP immunoreactive products expression had no significant effect. Magnolia compatibility 1:1 group ③ Polygala can alleviate the Polygala the gastrointestinal motility inhibition may be expressed with to reduce gastric antrum NO content, lower antral muscularis VIP immunoreactive product, enhanced duodenal AchE activity. Polygala Magnolia compatibility 1:1 and 1:2 group can alleviate to some extent the gastric mucosal injury caused by Polygala. ④ This study shows that the mechanism of action of each drug SOD, MDA, Na K-ATPase and Ca2-ATPase was no significant association. This study suggests that: the Magnolia with Wuyuan Zhi reduce gastric antrum tissue NO content down VIP expression may alleviate one of the main biological mechanism of gastric motility inhibition.

Related Dissertations

  1. Research on Mechanism Analysis and Modeling Simulation of Horizontal Well Tractor,P631.83
  2. Kunming Transient Population Community Present Situation, Question and Countermeasure Preliminary Study,C924.25
  3. Study on Wastewater Purification Effect of the Free Surface Wetland with Different Substrates and Plant Combination,X703
  4. Research of Multicast Protocol in Wireless Sensor Networks,TN929.5
  5. Study on Application of Non-phosphate Additive in Frozen Penaeus Vannamei and Mechanism of Water-holding,TS254.4
  6. Incentive Mechanism Research of the New Generation of Migrant Workers,F272.92
  7. Process and Mechanism of Eucalyptus Pulp Bleaching Catalysed by Cu2+/Co2+,TS745
  8. The Environmental-Friendly Method and Process of Removing Non-Cellulosic Matters from Bamboo for Textile Fiber,TS652
  9. The Research on Sulfate Leaching Properties of Cation-exchange Resin Used in Power Plants,TQ425.23
  10. Preparation and Anticorrosion Properties of Doped Polyaniline/Epoxy Coating,TQ637
  11. Practice and prospects of the Clean Development Mechanism in China,X38
  12. The Research on Mechanism of NaHCO3 in the Prevention and Cure of the Dothiorella Gregaria,S763.7
  13. Incidence and Mechanisms of Insecticide Resistances to Organophosphates and Carbamates in Colorado Potato Beetle, Leptinotarsa Decemlineata (Say),S435.32
  14. On the systematic construction of the Chinese Communist Party democracy,D262.11
  15. An Exploration of the Decision-making Mechanism for a Service-oriented Government,D035
  16. A Study of Theory and Practice in Interatcion between Primary Schools and Community Areas,G621
  17. College Teachers Flow Mechanism Research in Our Country,G645.1
  18. Structure the Institution of Chinese Archives Rendering the Service of Government Information Publicity,G273.5
  19. Curing Mechanism and Properties of Fluorenyl Epoxy Resin,TQ323.5
  20. Studies on the Key Technology of the Grinding Device for the Polymer Particles,TQ315
  21. Research on Management Efficiency and Mechanism of Soil-Water Erosion in Changting,S157

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology
© 2012 www.DissertationTopic.Net  Mobile