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Study on Compositions of Bamboo Leaf and Correlated Foundation Research

Author: XueYueQin
Tutor: YuanZuo
School: Zhejiang Forestry College
Course: Forest cultivation
Keywords: Bamboo leaves Quality assessment Flavonoid glycosides HPLC Element Amino acids Volatile oil
CLC: R284
Type: Master's thesis
Year: 2008
Downloads: 80
Quote: 0
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Abstract


Bamboo Poaceae perennial, bamboos (Bambusodeae), (Poacese), evergreen plants. As the research object in bamboo leaves, medicinal plants for the establishment of a comprehensive system evaluation system, of bamboo leaves ingredients flavonoid glycosides, volatile components, trace elements, amino acids, and other system research and analysis and obtain ideal results. The research on product development and quality control of bamboo leaves guiding significance. This paper studies the content is divided into four parts: the first part of the bamboo leaves flavonoids extraction method and extraction, purification process research. Rutin as index by UV spectrophotometric determination of different extraction methods the BLE on total flavonoids obtained ultrasonic extraction method is better than other methods; BLE on Diaion HP-20 different elution The parts of purification obtained 40% methanol part of total flavonoids highest, this method can be prepared a purity of 76% bamboo leaves flavonoids. The second part of the separation and preparation of reference substance of flavonoid glycosides. Prepared by column chromatography the four bamboo leaf flavonoid glycosides ingredients, as analyzed by HPLC with reference standards, and the antibacterial activity of experimental samples. The third part of the bamboo leaf flavonoid glycosides antibacterial activity. Antibacterial activity of experimental studies of flavonoid glycosides, drawn four flavonoid glycosides varying the degree of inhibition of fungi and bacteria. The fourth part of the bamboo leaves active ingredient analysis. Orientin glycosides as indicators orthogonal experimental samples orientin glucosinolate content by HPLC method, preferably ultrasound optimum extraction conditions: 10 times the amount of solvent extracted three times, each time 1min. As an indicator to four flavonoid glycosides using HPLC method for different production areas, different harvest seasons lophatheri flavonoid glycoside content analysis, derived Zhejiang the production lophatheri compared elsewhere flavonoid glycoside content mining lophatheri July flavonoids high glucosinolate content. Volatile Composition using different methods of extraction and GC-MS analysis of the comparative evaluation of different extraction methods, draw some valuable conclusions. Determination the different habitats lophatheri of trace elements and amino acid content, and cluster analysis to the 9 origin lophatheri based on trace elements and amino acid content of each divided into three categories, analyzed the extent they are similar. In this study, the initial establishment of bamboo leaves component analysis and quality evaluation of methods such as HPLC, GC / MS, AAS, for the first time orientin glycoside, iso-orientin glucosides, vitexin, at the same time as the marker of isovitexin compare the antibacterial activity by HPLC analysis of flavonoid glycosides lophatheri ingredients; nine different provinces in the country lophatheri flavonoid glycoside composition for the first time, trace elements, amino acid composition analysis and comparison. The bamboo leaves active ingredient through a variety of methods from multiple systems analysis, quality control methods and evaluation system for the rational use of bamboo leaves, provides a theoretical basis for the development and application of this natural resource.

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